Frankenne F, Rentier-Delrue F, Scippo M L, Martial J, Hennen G
J Clin Endocrinol Metab. 1987 Mar;64(3):635-7. doi: 10.1210/jcem-64-3-635.
Besides the hGH-N gene, which codes for the pituitary 22 and 20K GH variants, the human genome contains a second GH gene, namely the GH-V, which has been thought to be silent. We recently discovered a placental variant of human growth hormone (hPGH), which appears in maternal serum at mid-pregnancy and which rises in concentration thereafter to term. As hPGH and GH-V proteins display very similar characteristics, including a high affinity for hepatic GH receptors, they could be identical. To verify this hypothesis, we sought hGH-V mRNA in placenta. Hybridization experiments were performed between dot-blotted mRNA originating either from placenta or from one pituitary hGH secreting adenoma and synthetic polynucleotide probes corresponding to specific portions of the hGH-V or hGH-N gene sequences. The results indicate that the V gene is indeed expressed in the placenta and, at a very low level, in the pituitary adenoma. Therefore hPGH is most likely the expression product of the hGH-V gene.
除了编码垂体22K和20K生长激素变体的hGH-N基因外，人类基因组还包含第二个生长激素基因，即GH-V，人们一直认为它是沉默的。我们最近发现了一种人胎盘生长激素（hPGH）变体，它在孕中期出现在母体血清中，此后浓度持续上升直至足月。由于hPGH和GH-V蛋白具有非常相似的特性，包括对肝脏生长激素受体的高亲和力，它们可能是相同的。为了验证这一假设，我们在胎盘中寻找hGH-V mRNA。用来源于胎盘或一个垂体生长激素分泌腺瘤的点印迹mRNA与对应于hGH-V或hGH-N基因序列特定部分的合成多核苷酸探针进行杂交实验。结果表明，V基因确实在胎盘中表达，在垂体腺瘤中也有非常低水平的表达。因此，hPGH很可能是hGH-V基因的表达产物。
