Xiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China.
Changsha Cinotohi Technology Co., Ltd, No. 601, North Dongfanghong Road, Changsha, 410013, Hunan, China.
Anal Sci. 2024 Apr;40(4):599-607. doi: 10.1007/s44211-023-00489-0. Epub 2024 Jan 8.
Vitamin D (VD) is the main form of vitamin D and an essential nutrient for maintaining human life. Currently, traditional methods for detecting 25-hydroxyvitamin D3(25(OH)D) are complex and expensive. In this study, we constructed an accurate, sensitive, simple, and cost-effective label-free biosensor based on an aptamer for the detection of 25(OH)D. The aptamer-modified sulfhydryl adopted self-assembly as a way to stably immobilize at the glassy carbon electrode (GCE) surface modified by gold nanoparticles (AuNPs). Upon 25(OH)D binding to the aptamer, the complexes inhibit electron transfer at the electrode surface, leading to reduced [Fe(CN)] redox peak current. Consequently, the quantity of 25(OH)D that interacts with the electrode-bound aptamer correlates with the observed electric current response values. The Aptamer/AuNPs/GCE aptasensor achieved direct and highly sensitive detection of 25(OH)D over a wide concentration range (1.0-1000 nM), with a limit of detection of 1.0 nM. At the same time, other molecules with a similar structure, such as 25(OH)D, Vitamin D, and Vitamin D, had lower response interference than 25(OH)D. Therefore, this biosensor has great potential to become a portable diagnostic device for 25(OH)D.
维生素 D（VD）是维生素 D 的主要形式，是维持人体生命所必需的营养物质。目前，检测 25-羟维生素 D3（25（OH）D）的传统方法复杂且昂贵。在本研究中，我们构建了一种基于适体的准确、灵敏、简单且具有成本效益的无标记生物传感器，用于检测 25（OH）D。巯基修饰的适体采用自组装的方式稳定地固定在金纳米粒子（AuNPs）修饰的玻碳电极（GCE）表面。当 25（OH）D 与适体结合时，复合物会抑制电极表面的电子转移，导致[Fe（CN）] 氧化还原峰电流减小。因此，与电极结合的适体相互作用的 25（OH）D 的数量与观察到的电流响应值相关。Aptamer/AuNPs/GCE 适体传感器在较宽的浓度范围内（1.0-1000 nM）实现了对 25（OH）D 的直接和高灵敏度检测，检测限为 1.0 nM。同时，其他具有相似结构的分子，如 25（OH）D、维生素 D 和维生素 D，其响应干扰低于 25（OH）D。因此，这种生物传感器有很大的潜力成为一种用于 25（OH）D 的便携式诊断设备。
