Department of Chemistry, Birla Institute of Technology and Science-Pilani (Hyderabad Campus), Hyderabad, 500078, India.
Chem Asian J. 2024 Mar 1;19(5):e202301055. doi: 10.1002/asia.202301055. Epub 2024 Jan 30.
Human serum albumin (HSA) is an important biomarker that can be used for the early diagnosis of many diseases. In this work, a TICT probe bearing fused naphtho-furan scaffold (NPNF) was developed and employed in the selective turn-on sensing of HSA. The probe's selectivity towards HSA was observed using steady-state fluorescence experiments, with limit of quantitation in micromolar levels. NPNF's capability to exclusively detect HSA over BSA was further studied/rationalized using anisotropy and time-resolved studies. Molecular docking was used to shed light on the location of NPNF in the subdomain IB of HSA. The practical application of the probe was also demonstrated by the detection of HSA in urine and the HSA-assisted detection of cerium.
人血清白蛋白（HSA）是一种重要的生物标志物，可用于多种疾病的早期诊断。在这项工作中，开发了一种带有稠合萘并呋喃骨架的 TICT 探针（NPNF），并将其用于 HSA 的选择性开启传感。通过稳态荧光实验观察到探针对 HSA 的选择性，其定量限为微摩尔级。使用各向异性和时间分辨研究进一步研究/合理化 NPNF 仅在 BSA 上检测 HSA 的能力。分子对接用于阐明 NPNF 在 HSA 的亚域 IB 中的位置。通过检测尿液中的 HSA 和 HSA 辅助检测铈，还证明了探针的实际应用。
