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利用积雪草(Centella asiatica (L.) Urb.)的采后发光二极管(LED)技术,通过上调三萜类途径中的基因表达来提高积雪草苷含量。

Harnessing postharvest light emitting diode (LED) technology of (L.) Urb. to improve centelloside content by up-regulating gene expressions in the triterpenoid pathway.

作者信息

Kamol Puntitra, Nukool Wanrachon, Pumjaroen Sakuntala, Inthima Phithak, Kongbangkerd Anupan, Suphrom Nungruthai, Buddhachat Kittisak

机构信息

Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand.

Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, 65000, Thailand.

出版信息

Heliyon. 2023 Dec 17;10(1):e23639. doi: 10.1016/j.heliyon.2023.e23639. eCollection 2024 Jan 15.

Abstract

(L.) Urb. has wound healing, anti-inflammatory, cognitive improvement, and neuroprotective properties which have been attributed to its centelloside content. However, the quantities of these bioactive compounds are limited and vary due to genetic and environmental factors. Light qualities are known to enhance the production of secondary metabolites in several plant species, both preharvest and postharvest. In this study, fresh leaves of were subjected to different light emitting diode (LED) quality including white, dark, red, blue, and green to assess centelloside content, phytochemical constituents, and transcription level expression of triterpenoid biosynthesis genes. Results showed that white and blue LEDs significantly increased centelloside content in leaves at 3 days postharvest (dph) by 73 % over the control group at 0 dph. Blue LEDs stimulated the expression of triterpenoid biosynthesis genes including squalene synthase (), β-amyrin synthase (), and UDP gluclosyltransferase-73AH1 (; ), while different LED conditions gave diverse results. Red LED treatment triggered higher total flavonoid content (TFC) and total triterpenoid content (TTC) while white LEDs enhanced total triterpenoid content (TTC). Taken together, our findings suggest that postharvest under blue LEDs is a great approach to increase centelloside production of through gene up-regulation in triterpenoid pathway. Therefore, postharvest technology by LEDs serves as an effective tool for improving raw material quality for medicinal plant industries.

摘要

(L.) Urb.具有伤口愈合、抗炎、改善认知和神经保护特性,这些特性归因于其积雪草苷含量。然而,由于遗传和环境因素,这些生物活性化合物的含量有限且存在差异。已知光质会在收获前和收获后提高几种植物次生代谢产物的产量。在本研究中,对[植物名称未给出]的新鲜叶片进行了不同发光二极管(LED)光质处理,包括白色、黑暗、红色、蓝色和绿色,以评估积雪草苷含量、植物化学成分以及三萜生物合成基因的转录水平表达。结果表明,在收获后3天(dph),白色和蓝色LED显著提高了[植物名称未给出]叶片中的积雪草苷含量,比0 dph时的对照组高出73%。蓝色LED刺激了三萜生物合成基因的表达,包括鲨烯合酶([基因名称未给出])、β-香树脂醇合酶([基因名称未给出])和UDP-葡萄糖基转移酶-73AH1([基因名称未给出];[基因名称未给出]),而不同的LED条件产生了不同的结果。红色LED处理引发了更高的总黄酮含量(TFC)和总三萜含量(TTC),而白色LED提高了总三萜含量(TTC)。综上所述,我们的研究结果表明,收获后在蓝色LED下处理是通过上调三萜途径中的基因来增加[植物名称未给出]积雪草苷产量的好方法。因此,LED收获后技术是提高药用植物产业原材料质量的有效工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c46/10772125/7842d1afa6ee/ga1.jpg

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本文引用的文献

1
Therapeutic properties and pharmacological activities of asiaticoside and madecassoside: A review.
J Cell Mol Med. 2023 Mar;27(5):593-608. doi: 10.1111/jcmm.17635. Epub 2023 Feb 8.
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Pharmacological Effects of on Skin Diseases: Evidence and Possible Mechanisms.
Evid Based Complement Alternat Med. 2021 Nov 20;2021:5462633. doi: 10.1155/2021/5462633. eCollection 2021.
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Mitoprotective Effects of (L.) Urb.: Anti-Inflammatory and Neuroprotective Opportunities in Neurodegenerative Disease.
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