National Omics Center (NOC), National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Thailand Science Park, Pathum Thani, Thailand.
PLoS One. 2021 Nov 29;16(11):e0260468. doi: 10.1371/journal.pone.0260468. eCollection 2021.
Centella asiatica is rich in medical and cosmetic properties. While physiological responses of C. asiatica to light have been widely reported, the knowledge of the effects of light on its gene expression is sparse. In this study, we used RNA sequencing (RNA-seq) to investigate the expression of the C. asiatica genes in response to monochromatic red and blue light. Most of the differentially expressed genes (DEGs) under blue light were up-regulated but those under red light were down-regulated. The DEGs encoded for CRY-DASH and UVR3 were among up-regulated genes that play significant roles in responses under blue light. The DEGs involved in the response to photosystem II photodamages and in the biosynthesis of photoprotective xanthophylls were also up-regulated. The expression of flavonoid biosynthetic DEGs under blue light was up-regulated but that under red light was down-regulated. Correspondingly, total flavonoid content under blue light was higher than that under red light. The ABI5, MYB4, and HYH transcription factors appeared as hub nodes in the protein-protein interaction network of the DEGs under blue light while ERF38 was a hub node among the DEGs under red light. In summary, stress-responsive genes were predominantly up-regulated under blue light to respond to stresses that could be induced under high energy light. The information obtained from this study can be useful to better understand the responses of C. asiatica to different light qualities.
积雪草富含药用和美容特性。虽然已经广泛报道了积雪草对光的生理反应，但对光对其基因表达影响的了解还很匮乏。在这项研究中，我们使用 RNA 测序（RNA-seq）来研究单色红光和蓝光对积雪草基因表达的影响。大多数在蓝光下差异表达的基因（DEGs）上调，但在红光下下调。蓝光下上调的 DEGs 编码 CRY-DASH 和 UVR3，它们在蓝光响应中起着重要作用。参与光系统 II 光损伤反应和光保护类胡萝卜素生物合成的 DEGs 也上调。蓝光下类黄酮生物合成 DEGs 的表达上调，但红光下表达下调。相应地，蓝光下总类黄酮含量高于红光下。在蓝光下 DEGs 的蛋白质-蛋白质相互作用网络中，ABI5、MYB4 和 HYH 转录因子表现为枢纽节点，而 ERF38 是红光下 DEGs 的枢纽节点。总之，在蓝光下，应激响应基因主要上调，以应对高能量光下可能诱导的应激。本研究获得的信息有助于更好地理解积雪草对不同光质的响应。
