Could we use PD-1 and PD-L1 expression on lymphocytes and monocytes as predictive markers for prognosis of acute biliary pancreatitis?

PURPOSE

This study aimed to assess the significance of immunophenotyping and serum cytokines in predicting the clinical progression of acute biliary pancreatitis (ABP).

MATERIALS AND METHODS

Cytokine levels, T-helper, cytotoxic T, natural killer (NK) cells, monocytes, HLA-DR, and PD-1, as well as PDL-1 immune checkpoints, were measured in ABP patients at the time of diagnosis and compared with results from healthy volunteers. The study also compared leukocyte counts, hematocrit, immunophenotyping results, cytokine statuses, and PD-1, PDL-1 expression between healthy volunteers and ABP subgroups categorized by pancreatitis severity.

RESULTS

The study included 65 ABP patients and 20 healthy volunteers. Significant differences were observed between groups in hematocrit, leukocyte counts, total monocytes, lymphocytes, CD3 total T cells, CD4 Th cells, PD-1 expression on CD4 and CD8T lymphocytes, HLA-DR expression on CD14 monocytes, NK cells, PD-L1 expression on CD14+ monocytes, classical and intermediate monocytes, as well as levels of IL-6, IL-8, IL-10, IL-18, and IL-33 cytokines. Moderate correlations were found with lymphocyte counts, PD-1CD4 cells, PD-L1CD14 cells, and strong correlations with HLA-DRCD14 cells. Hematocrit, CD3 total T cells, NK cells, CD4PD-1  T cells, and CD8PD-1  T cells showed independent associations with the severity of ABP. Lymphocyte counts, CD14HLA-DR cells, CD14PD-L1 cells, CD4PD-1  T cells, classical, and intermediate monocytes exhibited the highest Area Under the Curve rates in determining organ failure.

CONCLUSIONS

Hematocrit, lymphocyte counts, CD14HLA-DR cells, CD14PD-L1 cells, and intermediate monocytes emerged as parameters most closely associated with the severity and these parameters could be useful in predicting the severity of ABP.