Xiao Tong, Li Yuhang, Yue Lin, Lu Zengkui, Yuan Chao, Song Yali, Yang Bohui, Liu Jianbin, Guo Tingting
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China.
Animals (Basel). 2023 Dec 29;14(1):127. doi: 10.3390/ani14010127.
SNPs associated with important traits of fine-wool sheep that were previously obtained through genome-wide association analysis screening were verified and analyzed. A total of 20 SNPs related to birth weight, bundle strength, cleaning rate, and fiber diameter were screened using whole-genome resequencing, and the SNPshot assay was used to detect and analyze polymorphisms. This study found that, among the 20 SNPs associated with important traits in Alpine Merino sheep, 8 were monomorphic and 12 were polymorphic, of which 6 showed moderate polymorphisms and 6 showed low polymorphisms. The heterozygosity of the 12 polymorphic loci ranged from 0.10 to 0.49, the effective number of alleles ranged from 1.11 to 1.98, and the polymorphic information content ranged from 0.09 to 0.37. The chi-square test showed that only RHPN2:g.42678119T>G and RALYL:g.90030866A>G were in Hardy-Weinberg disequilibrium ( < 0.05); the other loci were in equilibrium ( > 0.05). These SNPs associated with important traits in Alpine Merino sheep provide a theoretical basis for genomic selection and molecular design breeding.
对先前通过全基因组关联分析筛选获得的与细毛羊重要性状相关的单核苷酸多态性（SNPs）进行了验证和分析。利用全基因组重测序筛选出20个与出生体重、束强度、净毛率和纤维直径相关的SNPs，并采用SNPshot检测法对多态性进行检测和分析。本研究发现，在与高山美利奴羊重要性状相关的20个SNPs中，8个为单态，12个为多态，其中6个表现为中度多态，6个表现为低度多态。12个多态位点的杂合度范围为0.10至0.49，有效等位基因数范围为1.11至1.98，多态信息含量范围为0.09至0.37。卡方检验表明，只有RHPN2:g.42678119T>G和RALYL:g.90030866A>G处于哈迪-温伯格不平衡状态（<0.05）；其他位点处于平衡状态（>0.05）。这些与高山美利奴羊重要性状相关的SNPs为基因组选择和分子设计育种提供了理论依据。
