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基因标记辅助选择和蛋白质组学在秦川牛最佳肉质标准和体尺测量中的应用。

The application of gene marker-assisted selection and proteomics for the best meat quality criteria and body measurements in Qinchuan cattle breed.

作者信息

Abd El-Hack Mohamed E, Abdelnour Sameh A, Swelum Ayman A, Arif Muhammad

机构信息

Department of Poultry, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.

Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.

出版信息

Mol Biol Rep. 2018 Oct;45(5):1445-1456. doi: 10.1007/s11033-018-4211-y. Epub 2018 Jul 13.

Abstract

In the past few decades, enhancement of animal productivity has been gaining increasing attention among decisions-makers, politicians, mangers, and breeders, because of the increasing of world population and shortage of natural resources. The selection of high productivity animals is the main goal, through the application of genetic improvement programs. The use of molecular genetics has conferred significant breeding advantages over conventional breeding techniques. In this regard, many economic characteristics are controlled by a small number of multiple gene loci, each of which is responsible for trait diversity and hence they are referred to as quantitative trait loci (QTL). Single-nucleotide polymorphisms (SNPs), which have recently been discovered through DNA sequencing, are considered one of the most useful types of genetic marker. SNPs are found where different nucleotides occur at the same position in the DNA sequence. They are found in both coding and noncoding regions of the genome and are present at one SNP in every 1000 b. Strategies for the identification and application of markers are based on reference to examples of loci that can control various traits. Furthermore, markers for growth, body measurements, and meat quality traits are preferred, because they can be used to predict the performance of animals, via blood samples, in the first few days of animal life. Marker-assisted selection using SNPs, such asSIRT1, SIRT2, LPL, CRTC2, SIX4, UCPs, and ZBTB38as selection criteria of body measurements and meat traits in beef cattle, will be beneficial in selection and breeding programs. The proteomic is a novel marker and a new approache of biotechnology which increases the understanding of the biological processes, besides being a remarkable biomarker that interrelated to growth and meat quality traits. Proteomics is a vigorous tool as usage for deduces molecular processes between quality traits and muscle proteins, which are helpful in analyzing the mechanisms of biochemistry that influence quality. So they could be potential biomarker for some meat quality traits. Among them, Actin, Myosin, Heat shock proteins are used a novel approaches in the field of biotechnology to understand the proteomics changes. This review article highlights the novel findings on the potential use of MAS and proteomics as biomarker for the selection for meat quality and carcass traits in Qinchuan cattle breed.

摘要

在过去几十年里,由于世界人口增长和自然资源短缺,提高动物生产力越来越受到决策者、政治家、管理者和育种者的关注。选择高生产力动物是主要目标,通过应用遗传改良计划来实现。分子遗传学的应用相较于传统育种技术具有显著的育种优势。在这方面,许多经济性状由少数多个基因位点控制,每个基因位点负责性状多样性,因此它们被称为数量性状位点(QTL)。单核苷酸多态性(SNP)是最近通过DNA测序发现的,被认为是最有用的遗传标记类型之一。SNP出现在DNA序列中相同位置的不同核苷酸处。它们存在于基因组的编码区和非编码区,每1000个碱基中就有一个SNP。标记的鉴定和应用策略基于可控制各种性状的基因座实例。此外,生长、体尺和肉质性状的标记更受青睐,因为它们可用于在动物生命的最初几天通过血液样本预测动物的性能。使用SNP进行标记辅助选择,如将SIRT1、SIRT2、LPL、CRTC2、SIX4、UCPs和ZBTB38作为肉牛体尺和肉质性状的选择标准,将有利于选择和育种计划。蛋白质组学是一种新型标记和生物技术的新方法,除了作为与生长和肉质性状相关的重要生物标志物外,还能增进对生物过程的理解。蛋白质组学是一种有力工具,用于推断品质性状和肌肉蛋白质之间的分子过程,有助于分析影响品质的生物化学机制。因此,它们可能是某些肉质性状的潜在生物标志物。其中,肌动蛋白、肌球蛋白、热休克蛋白在生物技术领域被用作理解蛋白质组学变化的新方法。本文综述了标记辅助选择和蛋白质组学作为生物标志物在秦川牛品种肉质和胴体性状选择中的潜在应用的新发现。

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