一种基于单克隆抗体的灵敏且特异的酶联免疫吸附测定法，用于快速检测谷物和环境中的丙草胺。
A Sensitive and Specific Monoclonal Antibody Based Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Pretilachlor in Grains and the Environment.
作者信息
Zhang Linwei, Yin Xiaoyang, Yang Hongfei, Wen Hao, Han Shiyun, Pan Xiaoming, Li Huaming, Peng Dapeng
机构信息
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
出版信息
Foods. 2023 Dec 19;13(1):12. doi: 10.3390/foods13010012.
Pretilachlor is a chloroacetamide herbicide mainly used for weed and broadleaf weed control in rice, that is widely utilized in China. In order to detect the residue of pretilachlor in the environment and food, a highly sensitive and specific monoclonal antibody (mAb) against pretilachlor was prepared, and the half maximum inhibitory concentration (IC) of the monoclonal antibody was validated to be 31.47 ± 2.35 μg/L. An indirect competitive ELISA (ic-ELISA) based on the antibody with a linear range of 6.25100 μg/L was developed. The specificity of the antibody was explained by computer simulations and experimental validation. The mAb exhibited negligible cross-reactivity towards alachlor, acetochlor, propisochlor, butachlor, and metalaxyl, and the limits of detection (LOD) for pretilachlor in lake, rice, and soil samples were 4.835.23 μg/L. The recoveries of all samples were 78.3~91.3%. The reliability of the ic-ELISA method for residue detection of pretilachlor in the environment and grains was confirmed using high performance liquid chromatography.
丙草胺是一种主要用于防除稻田杂草和阔叶杂草的氯代乙酰胺类除草剂，在中国被广泛使用。为了检测环境和食品中丙草胺的残留量，制备了一种针对丙草胺的高灵敏度和特异性单克隆抗体（mAb），并验证该单克隆抗体的半数抑制浓度（IC）为31.47±2.35μg/L。基于该抗体开发了一种间接竞争酶联免疫吸附测定法（ic-ELISA），线性范围为6.25100μg/L。通过计算机模拟和实验验证解释了该抗体的特异性。该单克隆抗体对甲草胺、乙草胺、异丙草胺、丁草胺和甲霜灵的交叉反应可忽略不计，湖泊、水稻和土壤样品中丙草胺的检测限为4.835.23μg/L。所有样品的回收率为78.3~91.3%。使用高效液相色谱法证实了ic-ELISA法检测环境和谷物中丙草胺残留量的可靠性。
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