一种用于检测蔬菜、谷物和肉类中吡虫啉的敏感且特异的酶联免疫吸附测定法。
A sensitive and specific enzyme-linked immunosorbent assay for the detection of pymetrozine in vegetable, cereal, and meat.
机构信息
National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, PR China.
College of Life Sciences, Chongqing Normal University, Chongqing 401331, PR China.
出版信息
Food Chem. 2023 Aug 30;418:135949. doi: 10.1016/j.foodchem.2023.135949. Epub 2023 Mar 16.
Pymetrozine is a neonicotinoid insecticide with high efficacy against aphids and planthoppers, and has been used worldwide. To monitor its residue in food, a highly specific and sensitive monoclonal antibody (McAb) was prepared, and an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to detect pymetrozine, with a 50% inhibition value (IC) of 7.70 μg/L. The McAb showed little affinity for acetamiprid, hexazinone, metamitron, nitenpyram, metribuzin, and imidacloprid. The limits of detection (LOD) calculated from the analysis of broccoli, cabbage, wheat, maize, rice, chicken, fish, and crayfish samples were from 1.56 to 2.72 μg/kg and the average recoveries were from 81.25 to 103.19%. icELISA was confirmed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). These results demonstrated that the optimised icELISA is a convenient and effective analytical tool for monitoring pymetrozine residues in food.
吡虫啉是一种高效的新烟碱类杀虫剂，对蚜虫和飞虱有很高的防治效果，已在全球范围内使用。为了监测食品中的残留量，制备了一种高度特异性和灵敏性的单克隆抗体（McAb），并开发了间接竞争酶联免疫吸附测定（icELISA）来检测吡虫啉，其 50%抑制值（IC）为 7.70μg/L。该 McAb 对乙酰甲胺磷、己唑酮、灭蝇胺、噻虫嗪、噻虫啉和氯吡虫啉的亲和力很小。从分析花椰菜、白菜、小麦、玉米、水稻、鸡肉、鱼肉和小龙虾样品中计算出的检测限（LOD）范围为 1.56 至 2.72μg/kg，平均回收率范围为 81.25 至 103.19%。icELISA 得到了液相色谱-串联质谱（LC-MS/MS）的确认。这些结果表明，优化后的 icELISA 是一种方便、有效的分析工具，可用于监测食品中的吡虫啉残留量。
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