Tu Lanlan, Zeng Jihao, Bai Xue, Wu Ziyun, Wu Jinhong, Xu Shannan
Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Foods. 2024 Jan 2;13(1):158. doi: 10.3390/foods13010158.
Chlorella oil nanoliposomes (CO-NLP) were synthesized through ultrasonic injection with ethanol, and their physicochemical properties and hypolipidemic efficacy were systematically investigated. The results revealed that the mean particle size of CO-NLP was 86.90 nm and the encapsulation efficiency (EE) was 92.84%. Storage conditions at 4 °C were conducive to the stability of CO-NLP, maintaining an EE of approximately 90% even after 10 days of storage. The release profile of CO-NLP adhered more closely to the first-order kinetic model during in vitro assessments, exhibiting a slower release rate compared to free microalgae oil. In simulated in vitro digestion experiments, lipolytic reactions of CO-NLP were observed during intestinal digestion subsequent to nanoliposome administration. Notably, the inhibitory effect of CO-NLP on cholesterol esterase activity was measured at 85.42%. Additionally, the average fluorescence intensity of nematodes in the CO-NLP group was 52.17% lower than in the control group at a CO-NLP concentration of 500 μg/mL, which suggests a pronounced lipid-lowering effect of CO-NLP. Therefore, the CO-NLP exhibited characteristics of small and uniform particle size, elevated storage stability, gradual release during intestinal digestion, and a noteworthy hypolipidemic effect. These findings designate CO-NLP as a novel lipid-lowering active product, demonstrating potential for the development of functional foods.
通过乙醇超声注射法合成了小球藻油纳米脂质体（CO-NLP），并对其理化性质和降血脂功效进行了系统研究。结果表明，CO-NLP的平均粒径为86.90nm，包封率（EE）为92.84%。4℃的储存条件有利于CO-NLP的稳定性，即使储存10天后，EE仍能保持在约90%。在体外评估中，CO-NLP的释放曲线更符合一级动力学模型，与游离微藻油相比，释放速率较慢。在模拟体外消化实验中，纳米脂质体给药后，在肠道消化过程中观察到CO-NLP的脂解反应。值得注意的是，测得CO-NLP对胆固醇酯酶活性的抑制作用为85.42%。此外，在CO-NLP浓度为500μg/mL时，CO-NLP组线虫的平均荧光强度比对照组低52.17%，这表明CO-NLP具有显著的降血脂作用。因此，CO-NLP表现出粒径小且均匀、储存稳定性高、在肠道消化过程中逐渐释放以及显著的降血脂作用等特点。这些发现表明CO-NLP是一种新型的降血脂活性产品，具有开发功能性食品的潜力。
