Stem Cell Research Centre, Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226014, India.
National Institute of Animal Biotechnology (NIAB), Hyderabad 500032, India.
Cells. 2023 Dec 24;13(1):41. doi: 10.3390/cells13010041.
The selection of an appropriate scaffold is imperative for the successful development of alternative animal protein in the form of cultured meat or lab-grown meat. Decellularized tissues have been suggested as a potential scaffold for cultured meat production owing to their capacity to support an optimal environment and niche conducive to cell proliferation and growth. This approach facilitates the systematic development of 3D tissues in the laboratory. Decellularized scaffold biomaterials have characteristics of high biocompatibility, biodegradation, and various bioactivities, which could potentially address the limitations associated with synthetic bio-scaffold materials. The present study involved the derivation and characterization of a decellularized scaffold from mushroom tissue following subsequent assessment of the scaffold's capacity to support myogenic differentiation. Mushroom sections were soaked in nuclease and detergent solution for 4 days. Furthermore, decellularization was confirmed by histology and DAPI staining, which showed the removal of cellular components and nuclei. Myoblast cells were seeded onto decellularized tissue, which exhibited excellent cytocompatibility and promoted myogenic growth and differentiation. The study's findings can serve as a foreground for the generation of an edible and natural scaffold for producing a safe and disease-free source of alternative animal protein, potentially reducing the burden on the health sector caused by conventional animal protein production and consumption.
选择合适的支架对于成功开发替代动物蛋白（以培养肉或实验室培育肉的形式）至关重要。由于脱细胞组织能够支持有利于细胞增殖和生长的最佳环境和小生境，因此被提议作为培养肉生产的潜在支架。这种方法有助于在实验室中系统地开发 3D 组织。脱细胞支架生物材料具有高生物相容性、可生物降解性和多种生物活性等特点，可能解决与合成生物支架材料相关的局限性。本研究从蘑菇组织中提取和表征了脱细胞支架，随后评估了该支架支持肌原性分化的能力。蘑菇切片在核酸酶和去污剂溶液中浸泡了 4 天。此外，通过组织学和 DAPI 染色证实了脱细胞，显示出细胞成分和细胞核的去除。肌母细胞接种到脱细胞组织上，表现出优异的细胞相容性，并促进肌原性生长和分化。该研究的结果可以为生产安全无病的替代动物蛋白的可食用和天然支架提供前景，可能会减轻传统动物蛋白生产和消费给卫生部门带来的负担。
