Jang Woong Sik, Lee Junmin, Park Seoyeon, Lim Chae Seung, Kim Jeeyong
Departments of Emergency Medicine, College of Medicine, Korea University Guro Hospital, Seoul 08308, Republic of Korea.
Departments of Laboratory Medicine, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Diagnostics (Basel). 2023 Dec 28;14(1):73. doi: 10.3390/diagnostics14010073.
Counting CD4+ T lymphocytes using flow cytometry is a standard method for monitoring patients with HIV infections. Simpler and cheaper alternatives to flow cytometry are in high demand because getting access to flow cytometers is difficult or impossible in resource-limited settings. We evaluated the performance of the Microscanner Plus, a simple and automated image-based cell counter, in determining CD4 counts against a flow cytometer. CD4 count results of the Microscanner Plus and flow cytometer were compared using samples from 47 HIV-infected patients and 87 healthy individuals. All CV% for precision and reproducibility tests were less than 10%. The Microscanner Plus's lowest detectable CD4 count was determined to be 15.27 cells/µL of whole blood samples. The correlation coefficient (R) between Microscanner Plus and flow cytometry for CD4 counting in 134 clinical samples was very high, at 0.9906 ( < 0.0001). The automated Microscanner Plus showed acceptable analytical performance for counting CD4+ T lymphocytes and may be particularly useful for monitoring HIV patients in resource-limited settings.
使用流式细胞术计数CD4 + T淋巴细胞是监测HIV感染患者的标准方法。由于在资源有限的环境中难以或无法使用流式细胞仪，因此对更简单、更便宜的流式细胞术替代方法有很高的需求。我们评估了Microscanner Plus（一种简单的基于图像的自动细胞计数器）相对于流式细胞仪测定CD4计数的性能。使用来自47名HIV感染患者和87名健康个体的样本，比较了Microscanner Plus和流式细胞仪的CD4计数结果。精度和重现性测试的所有CV％均小于10％。Microscanner Plus的最低可检测CD4计数被确定为全血样本15.27个细胞/μL。134份临床样本中，Microscanner Plus与流式细胞术在CD4计数方面的相关系数（R）非常高，为0.9906（<0.0001）。自动化的Microscanner Plus在计数CD4 + T淋巴细胞方面显示出可接受的分析性能，对于在资源有限的环境中监测HIV患者可能特别有用。
