Research Product Department, R&D Center, Glac Biotech Co., Ltd., Tainan City 744, Taiwan.
Functional Investigation Department, R&D Center, Glac Biotech Co., Ltd., Tainan City 744, Taiwan.
Nutrients. 2023 Dec 29;16(1):126. doi: 10.3390/nu16010126.
subsp. BLI-02, ET-66, LPL28, and TYCA06, isolated from healthy breast milk, miso, and the healthy human gut, were assessed for safety in this study. BLI-02, LPL28, TYCA06, and ET-66 exhibited no antibiotic resistance and mutagenic activity in the Ames test at the highest dosage (5000 μg/plate). No genotoxicity was observed in micronucleus and chromosomal aberration assays in rodent spermatogonia at the maximum dosage of 10 g/kg body weight (BW). No acute and sub-chronic toxicity occurred in mice and rats at the maximum tested dosage of 10 g/kg BW and 1.5 g/kg BW, respectively. The lyophilized powder of these strains survived a low pH and high bile salt environment, adhering strongly to Caco-2 cells. Unique antimicrobial activities were noted in these strains, with BLI-02 demonstrating the best growth inhibition against , LPL28 exhibiting the best growth inhibition against , and ET-66 showing the best growth inhibition against . Based on the present study, the lyophilized powder of these four strains appears to be a safe probiotic supplement at tested dosages. It should be applicable for clinical or healthcare applications.
在本研究中，评估了从健康母乳、味增和健康人体肠道中分离出的 subsp. BLI-02、ET-66、LPL28 和 TYCA06 的安全性。BLI-02、LPL28、TYCA06 和 ET-66 在最高剂量（5000μg/平板）的 Ames 试验中均无抗生素耐药性和诱变活性。在最大剂量为 10 g/kg 体重（BW）的啮齿动物精原细胞微核和染色体畸变试验中未观察到遗传毒性。在最大测试剂量为 10 g/kg BW 和 1.5 g/kg BW 的小鼠和大鼠中未发生急性和亚慢性毒性。这些菌株的冻干粉末能够在低 pH 值和高胆汁盐环境中存活，并强烈附着于 Caco-2 细胞。这些菌株具有独特的抗菌活性，BLI-02 对 的生长抑制作用最好，LPL28 对 的生长抑制作用最好，ET-66 对 的生长抑制作用最好。基于本研究，这些 4 株菌的冻干粉末在测试剂量下似乎是一种安全的益生菌补充剂。它应该适用于临床或医疗保健应用。
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