Buonanno Annalisa, Imparato Marianna, Maione Angela, Carraturo Federica, Galdiero Emilia, Guida Marco, de Alteriis Elisabetta
Department of Biology, University of Naples "Federico II", 80126 Naples, Italy.
BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples "Federico II", 80055 Portici, Italy.
J Fungi (Basel). 2025 Feb 14;11(2):147. doi: 10.3390/jof11020147.
There is an increasing interest in yeasts isolated from natural sources to be used as probiotics. -based probiotics have been proposed as a valid alternative to the conventional drug therapy for the prevention and treatment of vulvovaginal candidiasis, also considering the resistance of some strains to many antifungals. Here, we isolated from an artisanal sourdough a new yeast strain which was identified as and assessed its probiotic and safety properties, which resulted in comparable properties to all those exhibited by the commercial probiotic . Then, we checked the antagonistic activity of the new isolate against some clinical fluconazole resistant strains, showing its ability to inhibit filamentation, biofilm formation, and the adhesion of to vaginal epithelial A-431 cells. Also, reduced the cell damage provoked by and the expression of and genes. On the whole, our results enlarge the spectrum of the beneficial properties of the food-grade yeast showing for the first time its biotherapeutic potential against .
人们越来越关注从天然来源分离出的酵母作为益生菌的应用。基于此，考虑到一些菌株对多种抗真菌药物的耐药性，基于酵母的益生菌已被提议作为预防和治疗外阴阴道念珠菌病的传统药物治疗的有效替代方案。在这里，我们从一种手工制作的酸面团中分离出一种新的酵母菌株，鉴定为，并评估了其益生菌和安全性特性，其结果与商业益生菌所表现出的所有特性相当。然后，我们检测了新分离株对一些临床氟康唑耐药菌株的拮抗活性，结果表明它能够抑制丝状化、生物膜形成以及对阴道上皮A - 431细胞的黏附。此外，还减少了由引起的细胞损伤以及和基因的表达。总体而言，我们的结果拓宽了食品级酵母的有益特性范围，首次展示了其针对的生物治疗潜力。
