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乌克兰产药草中多酚和三萜类化合物含量的物候变化

Phenological Variations in the Content of Polyphenols and Triterpenoids in Herb Originating from Ukraine.

作者信息

Ivanauskas Liudas, Uminska Kateryna, Gudžinskas Zigmantas, Heinrich Michael, Georgiyants Victoriya, Kozurak Alla, Mykhailenko Olha

机构信息

Department of Analytical and Toxicological Chemistry, Lithuanian University of Health Sciences, A. Mickevičiaus Str. 9, 44307 Kaunas, Lithuania.

Zhytomyr Basic Pharmaceutical Professional College, Chudnivska Str. 99, 10005 Zhytomyr, Ukraine.

出版信息

Plants (Basel). 2023 Dec 31;13(1):120. doi: 10.3390/plants13010120.

Abstract

The composition of secondary metabolites undergoes significant changes in plants depending on the growth phase and the influence of environmental factors. Therefore, it is important to determine the harvesting time of plant material for the optimum secondary metabolite profile and therapeutic activity of the primary material. The shoots of are used as a healing tea due to the presence of polyphenolic compounds. The aim of this study was to assess the composition of phenolic compounds and triterpenoid saponins in leaves and flowers and to estimate the dynamics of their content depending on the flowering phase. Qualitative and quantitative characterisation of polyphenols and triterpenoids in samples from Ukraine of three flowering phases were performed using the high-performance liquid chromatography photo diode array (HPLC-PDA) method. During the present study, 13 polyphenolic compounds and seven triterpenoids were identified in the plant material. It was noted that the largest content and the best polyphenol profile was in late flowering. The most important polyphenolic compounds in the plant material were chlorogenic acid, hyperoside, isoquercitin, and oenothein B. The triterpenoid profile was at its maximum during mass flowering, with corosolic and ursolic acids being the dominant metabolites. The results of the analysis revealed that the quantity of many of the tested metabolites in the raw material of is dependent on the plant organ and flowering phase. The largest content of most metabolites in the leaves was in late flowering. In the flowers, the quantity of the metabolites studied was more variable, but decreased during mass flowering and increased significantly again in late flowering. The results show that raw material is a potential source of oenothein B and triterpenoids.

摘要

次生代谢产物的组成在植物中会根据生长阶段和环境因素的影响而发生显著变化。因此,确定植物材料的收获时间对于获得最佳的次生代谢产物谱和主要材料的治疗活性非常重要。由于含有多酚类化合物,[植物名称]的嫩枝被用作药茶。本研究的目的是评估[植物名称]叶片和花朵中酚类化合物和三萜皂苷的组成,并根据开花阶段估计其含量动态。使用高效液相色谱光电二极管阵列(HPLC-PDA)方法对来自乌克兰的三个开花阶段的[植物名称]样品中的多酚和三萜进行了定性和定量表征。在本研究中,在植物材料中鉴定出了13种多酚类化合物和7种三萜。值得注意的是,开花后期的含量最高且多酚谱最佳。植物材料中最重要的多酚类化合物是绿原酸、金丝桃苷、异槲皮苷和月见草素B。三萜谱在盛花期达到最大值,其中科罗索酸和熊果酸是主要代谢产物。分析结果表明,[植物名称]原料中许多被测代谢产物的含量取决于植物器官和开花阶段。叶片中大多数代谢产物的最大含量出现在开花后期。在花朵中,所研究的代谢产物数量变化更大,但在盛花期减少,在开花后期又显著增加。结果表明,[植物名称]原料是月见草素B和三萜的潜在来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/88e0/10781012/82c67817f03b/plants-13-00120-g001.jpg

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