Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland.
Chair and Department of Human Physiology, Medical University of Lublin, Radziwiłłowska 11, 20-080 Lublin, Poland.
Molecules. 2022 Feb 2;27(3):1006. doi: 10.3390/molecules27031006.
The biological activity of an in vitro digested infusion of (fireweed) was examined in a model system of intestinal epithelial and colon cancer tissues. The content of selected phenolic compounds in the digested aqueous extract of fireweed was determined using HPLC-ESI-QTOF-MS/MS. Biological activity was examined using the human colon adenocarcinoma cell lines HT-29 and CaCo-2 and the human colon epithelial cell line CCD 841 CoTr. Cytotoxicity was assessed by an MTT assay, a Neutral Red uptake assay, May-Grünwald-Giemsa staining, and a label-free Electric Cell-Substrate Impedance Sensing cytotoxicity assay. The effect of the infusion on the growth of selected intestinal bacteria was also examined. The extract inhibited the growth of intestinal cancer cells HT-29. This effect can be attributed to the activity of quercetin and kaempferol, which were the most abundant phenolic compounds found in the extract after in vitro digestion. The cytotoxicity of the fireweed infusion was dose-dependent. The highest decrease in proliferation (by almost 80%) compared to the control was observed in HT-29 line treated with the extract at a concentration of 250 μg/mL. The fireweed infusion did not affect the growth of beneficial intestinal bacteria, but it did significantly inhibit . The cytotoxic effect of the fireweed extract indicates that it does not lose its biological activity after in vitro digestion. It can be concluded that the fireweed infusion has the potential to be used as a supporting agent in colon cancer therapy.
对（金莲花）体外消化液的生物活性进行了研究，该模型系统包括肠上皮组织和结肠癌组织。采用 HPLC-ESI-QTOF-MS/MS 法测定了金莲花体外消化水提物中选定酚类化合物的含量。采用人结肠腺癌细胞系 HT-29 和 CaCo-2 以及人结肠上皮细胞系 CCD 841 CoTr 研究了生物活性。通过 MTT 测定法、中性红摄取测定法、May-Grünwald-Giemsa 染色和无标记电细胞-底物阻抗传感细胞毒性测定法评估细胞毒性。还研究了金莲花浸液对选定肠道细菌生长的影响。该提取物抑制了肠道癌细胞 HT-29 的生长。这种作用可以归因于槲皮素和山柰酚的活性，它们是体外消化后提取物中含量最丰富的酚类化合物。金莲花浸液的细胞毒性呈剂量依赖性。与对照相比，在浓度为 250μg/ml 的提取物处理的 HT-29 细胞中，增殖减少了近 80%。金莲花浸液对有益肠道细菌的生长没有影响，但对显著抑制。金莲花提取物的细胞毒性表明，它在体外消化后不会失去其生物活性。可以得出结论，金莲花浸液有可能作为结肠癌治疗的辅助剂。
