AceBiome Inc., Seoul 06164, Republic of Korea.
R&D Center, AceBiome Inc., Daejeon 34013, Republic of Korea.
Int J Mol Sci. 2023 Dec 21;25(1):162. doi: 10.3390/ijms25010162.
Owing to increasing air pollution due to industrial development, fine dust has been associated with threatening public health. In particular, ultrafine urban particulate matter (uf-UP, PM 0.1) can easily enter our bodies, causing inflammation-related diseases. Therefore, in the present study, we evaluated the effects of hydrothermal extracts of and its bioactive compound, loliolide, on uf-UP-induced inflammation as a potential treatment strategy for retinal disorders. Human retinal pigment epithelial cells (ARPE-19) stimulated with TNF-α or uf-UPs were treated with extract and loliolide. extracts exhibited anti-inflammatory effects on uf-UP-induced inflammation without cell toxicity through downregulating the mRNA expression of , , , and . UPLC-QTOF/MS analysis confirmed that the hydrothermal extract of contained loliolide, which has anti-inflammatory effects. Loliolide effectively reduced the mRNA expression and production of proinflammatory chemokines () and cytokines ( and ) by downregulating the MAPK/NF-ĸB signaling pathway on TNF-α-stimulated inflammatory ARPE-19 cells. These effects were further confirmed in inflammatory ARPE-19 cells after stimulation with uf-UPs. Collectively, these results suggested the application of as a functional ingredient for treating ocular disorders caused by particular matters.
由于工业发展导致的空气污染加剧，细颗粒物已与威胁公众健康有关。特别是，超细微城市颗粒物（uf-UP，PM0.1）很容易进入我们的身体，引发炎症相关疾病。因此，在本研究中，我们评估了水热提取物及其生物活性化合物，芫荽内酯，对 uf-UP 诱导的炎症的影响，作为治疗视网膜疾病的潜在策略。用 TNF-α或 uf-UPs 刺激人视网膜色素上皮细胞（ARPE-19）后，用 提取物和芫荽内酯进行处理。提取物对 uf-UP 诱导的炎症具有抗炎作用，而没有细胞毒性，通过下调 、 、 和 的 mRNA 表达。UPLC-QTOF/MS 分析证实水热提取的芫荽内酯具有抗炎作用。芫荽内酯通过下调 MAPK/NF-ĸB 信号通路，有效降低 TNF-α刺激的炎性 ARPE-19 细胞中促炎趋化因子（ 和 ）和细胞因子（ 和 ）的 mRNA 表达和产生。在 uf-UP 刺激后的炎性 ARPE-19 细胞中进一步证实了这些作用。综上所述，这些结果表明可以将 用作治疗特定物质引起的眼部疾病的功能性成分。
