Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Science & Biotechnology, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2023 Dec 23;25(1):258. doi: 10.3390/ijms25010258.
This study explored the physicochemical properties and prebiotic activities of glucans and oligoglucans. Oligoglucans were obtained through the fermentation of CICC6055 and the glucansucrase of strain CICC6055, while glucans were obtained only through fermentation. Thin-layer chromatography and high-performance liquid chromatography identified enzymatically synthesized oligoglucans with a higher yield. Differential scanning calorimetry and derivative thermogravimetry analyses revealed the heat resistance of the glucans and oligoglucans at 280-300 °C. Fourier transform-infrared spectroscopy and nuclear magnetic resonance analyses demonstrated that their main chains were linked with α-1,6-glycosidic bonds accompanied by glucose residue branching. In vitro fermentation experiments demonstrated that they not only improved the contents of short-chain fatty acids but also raised the abundance of predominant flora, such as , , , and . These results implicate glucansucrase as an efficacious tool for the enzyme synthesis of oligoglucans. Furthermore, both polysaccharides with different degrees of polymerization may be beneficial in maintaining a healthy human gut.
本研究探索了葡聚糖和低聚葡聚糖的物理化学性质和益生元活性。低聚葡聚糖通过 CICC6055 的发酵和 CICC6055 菌株的葡聚糖蔗糖酶获得，而葡聚糖仅通过发酵获得。薄层层析和高效液相色谱法鉴定了酶法合成的低聚葡聚糖具有更高的产率。差示扫描量热法和导数热重分析表明，葡聚糖和低聚葡聚糖在 280-300°C 下具有耐热性。傅里叶变换-红外光谱和核磁共振分析表明，它们的主链通过α-1,6-糖苷键连接，同时伴有葡萄糖残基分支。体外发酵实验表明，它们不仅提高了短链脂肪酸的含量，还增加了主要菌群的丰度，如 、 、 和 。这些结果表明葡聚糖蔗糖酶是一种有效的低聚葡聚糖酶合成工具。此外，具有不同聚合度的多糖都可能有益于维持健康的人类肠道。
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