Institute of Medical Sciences, College of Medical Sciences, University of Rzeszow, 35-959 Rzeszow, Poland.
Centre for Innovative Research in Medical and Natural Sciences, Medical College of Rzeszów University, 35-310 Rzeszow, Poland.
Int J Mol Sci. 2023 Dec 28;25(1):417. doi: 10.3390/ijms25010417.
Circular RNAs (circRNAs) are noncoding molecules and are generated through back splicing, during which the 5' and 3' ends are covalently joined. Consequently, the lack of free ends makes them stable and resistant to exonucleases, and they become more suitable biomarkers than other noncoding RNAs. The aim of the study was to find an association between selected circRNAs and disease activity in patients with RA. A total of 71 subjects, 45 patients with RA and 26 healthy controls (HCs), were enrolled. In the RA group, 24 patients had high disease activity (DAS-28-ESR > 5.1) and 21 individuals were in remission (DAS-28-ESR ≤ 2.6). The cell line SW982 was used to evaluate the biological function of circ_0005567. The concentration of circ_0005567 in RA patients was elevated compared to HCs (median, 177.5 [lower-upper quartile, 83.13-234.6] vs. 97.83 [42.03-145.4], = 0.017). Patients with high disease activity had a higher concentration of circ_0005567 than the control group (185.4 [112.72-249.25] vs. 97.83 [42.03-145.4], = 0.015). In the cell line model, we found an association between circ_0005567 and miR-194-5p concentration and increased expression of mRNAs that may be related to cell proliferation. The plasma concentration of circ_0005567 may be a new potential biomarker associated with disease activity in patients with RA.
环状 RNA（circRNAs）是一类非编码分子，通过反向剪接产生，在此过程中，5'和 3'末端通过共价键连接。因此，缺少游离末端使得它们稳定且不易被核酸外切酶降解，并且它们比其他非编码 RNA 更适合作为生物标志物。本研究旨在探讨所选环状 RNA 与 RA 患者疾病活动度之间的关系。共纳入 71 例受试者，45 例 RA 患者和 26 例健康对照（HC）。在 RA 组中，24 例患者疾病活动度较高（DAS-28-ESR＞5.1），21 例患者处于缓解期（DAS-28-ESR≤2.6）。使用 SW982 细胞系评估 circ_0005567 的生物学功能。与 HC 相比，RA 患者的 circ_0005567 浓度升高（中位数，177.5[下四分位数-上四分位数，83.13-234.6]比 97.83[42.03-145.4]， = 0.017）。疾病活动度较高的患者的 circ_0005567 浓度高于对照组（185.4[112.72-249.25]比 97.83[42.03-145.4]， = 0.015）。在细胞系模型中，我们发现 circ_0005567 与 miR-194-5p 浓度之间存在关联，并且与细胞增殖相关的 mRNAs 表达增加。血浆中环状 RNA（circRNA）的浓度可能是与 RA 患者疾病活动度相关的新的潜在生物标志物。
Zotero 插件
