Podgórski Rafał, Cieśla Marek, Podgórska Dominika, Bajorek Wojciech, Płonka Artur, Czarny Wojciech, Trybulski Robert, Król Paweł
Department of Biochemistry, Institute of Medical Sciences, Medical College of Rzeszow University, 35-959 Rzeszow, Poland.
Centre for Innovative Research in Medical and Natural Sciences, Medical College of Rzeszow University, 35-310 Rzeszow, Poland.
J Clin Med. 2022 Jan 5;11(1):263. doi: 10.3390/jcm11010263.
A deeper insight into the mechanisms responsible for athlete performance that may serve as specific and detailed training indicators is still desired, because conventionally used biomarkers provide limited information about the adaptive processes that occur during exercise. The objective of our study was to assess insulin-like growth factor 1 receptors () gene expression and evaluate plasma concentration of selected microRNAs (miRNAs) during a 10-week training period (sampling times: week 1, 4, 7, and 10) in a group of 12 professional female volleyball players. Circulating miRNAs (miR-223, miR-320a, and miR-486) with established concentration in plasma and documented association with the IGF1 signaling pathway, which is involved in muscle development and recovery, were tested. The levels of analyzed miRNAs, tested by one-way ANOVA, were significantly different between four training periods during a 10-week training cycle (miR-223 < 0.0001, miR-320a = 0.00021, miR-486 = 0.0037, respectively). The levels of also appeared to be different ( = 0.00092), and their expression showed a trend to increase between the first and third periods. In the fourth period, the expression decreased, although it was higher compared with the baseline. Correlations between concentration levels of miR-223 and miR-320a (rs = 0.54, < 0.001), as well as between miR-320a and miR-486 (rs = 0.73, < 0.001) were also found. In the fourth period, a negative correlation between miR-223 plasma level and leucocyte expression was found (rs = -0.63, = 0.028). Multiple linear regression analysis showed that miR-320a ( = 0.024) and creatine kinase ( = 0.028) had the greatest impact on the expression levels of the gene. Future studies are required to define whether these miRNAs, especially miR-320a, as well as expression could be useful biomarkers of physiological changes during exercise and to discover their detailed biological roles in mode-specific exercise training adaptations of professional athletes.
人们仍希望能更深入地了解影响运动员表现的机制，这些机制可作为具体而详细的训练指标，因为传统使用的生物标志物提供的关于运动过程中发生的适应性过程的信息有限。我们研究的目的是评估12名职业女排运动员在为期10周的训练期（采样时间：第1、4、7和10周）内胰岛素样生长因子1受体（IGF1R）基因表达，并评估所选 microRNA（miRNA）的血浆浓度。检测了血浆中已确定浓度且与参与肌肉发育和恢复的IGF1信号通路有记录关联的循环miRNA（miR-223、miR-320a和miR-486）。通过单因素方差分析测试的分析miRNA水平在10周训练周期的四个训练期之间有显著差异（miR-223 P<0.0001，miR-320a P = 0.00021，miR-486 P = 0.0037）。IGF1R水平似乎也有所不同（P = 0.00092），其表达在第一和第三期之间呈上升趋势。在第四期，表达下降，尽管仍高于基线水平。还发现miR-223和miR-320a浓度水平之间存在相关性（rs = 0.54，P<0.001），以及miR-320a和miR-486之间存在相关性（rs = 0.73，P<0.001）。在第四期，发现miR-223血浆水平与白细胞IGF1R表达之间存在负相关（rs = -0.63，P = 0.028）。多元线性回归分析表明，miR-320a（P = 0.024）和肌酸激酶（P = 0.028）对IGF1R基因表达水平影响最大。未来需要开展研究来确定这些miRNA，尤其是miR-320a，以及IGF1R表达是否可作为运动过程中生理变化的有用生物标志物，并发现它们在职业运动员特定模式运动训练适应中的详细生物学作用。
