State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing 400716, China.
College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400716, China.
Genes (Basel). 2021 Jul 2;12(7):1036. doi: 10.3390/genes12071036.
Although ignored in the past, with the recent deepening of research, significant progress has been made in the field of non-coding RNAs (ncRNAs). Accumulating evidence has revealed that microRNA (miRNA) response elements regulate RNA. Long ncRNAs, circular RNAs, pseudogenes, miRNAs, and messenger RNAs (mRNAs) form a competitive endogenous RNA (ceRNA) network that plays an essential role in cancer and cardiovascular, neurodegenerative, and autoimmune diseases. Gastric cancer (GC) is one of the most common cancers, with a high degree of malignancy. Considerable progress has been made in understanding the molecular mechanism and treatment of GC, but GC's mortality rate is still high. Studies have shown a complex ceRNA crosstalk mechanism in GC. lncRNAs, circRNAs, and pseudogenes can interact with miRNAs to affect mRNA transcription. The study of the involvement of ceRNA in GC could improve our understanding of GC and lead to the identification of potential effective therapeutic targets. The research strategy for ceRNA is mainly to screen the different miRNAs, lncRNAs, circRNAs, pseudogenes, and mRNAs in each sample through microarray or sequencing technology, predict the ceRNA regulatory network, and, finally, conduct functional research on ceRNA. In this review, we briefly discuss the proposal and development of the ceRNA hypothesis and the biological function and principle of ceRNAs in GC, and briefly introduce the role of ncRNAs in the GC's ceRNA network.
尽管过去被忽视，但随着最近研究的深入，非编码 RNA（ncRNA）领域取得了重大进展。越来越多的证据表明，微小 RNA（miRNA）反应元件调节 RNA。长 ncRNA、环状 RNA、假基因、miRNA 和信使 RNA（mRNA）形成竞争性内源 RNA（ceRNA）网络，在癌症和心血管、神经退行性和自身免疫性疾病中发挥着重要作用。胃癌（GC）是最常见的癌症之一，恶性程度高。对 GC 的分子机制和治疗的理解取得了相当大的进展，但 GC 的死亡率仍然很高。研究表明，GC 中存在复杂的 ceRNA 串扰机制。lncRNA、circRNA 和假基因可以与 miRNA 相互作用，影响 mRNA 转录。研究 ceRNA 在 GC 中的作用可能会加深我们对 GC 的理解，并为确定潜在有效的治疗靶点提供帮助。ceRNA 的研究策略主要是通过微阵列或测序技术筛选每个样本中的不同 miRNA、lncRNA、circRNA、假基因和 mRNA，预测 ceRNA 调控网络，最后对 ceRNA 进行功能研究。在这篇综述中，我们简要讨论了 ceRNA 假说的提出和发展，以及 ceRNAs 在 GC 中的生物学功能和原理，并简要介绍了 ncRNA 在 GC 的 ceRNA 网络中的作用。
