Aggarwal A, Cuna W, Haque A, Dissous C, Capron A
Immunology. 1985 Apr;54(4):655-63.
Several monoclonal antibodies were produced following the immunization of mice with infective larvae of Brugia malayi. One of these gives a positive fluorescence reaction on the surface of B. malayi microfilariae and this particular monoclonal antibody (IgM isotype) was able to mediate mouse peritoneal macrophage adherence to, and killing of, B. malayi microfilariae in vitro. Adherence and killing were enhanced by fresh normal mouse serum, suggesting a role for complement. When the same monoclonal antibody was passively transferred to mice harbouring microfilariae in their circulation, a complete clearance of microfilariae was observed in 70% of the animals. This monoclonal antibody was able to recognize antigenic determinants (of 110,000 MW) present on the surface of B. malayi microfilariae by radioimmunoprecipitation.
用马来布鲁线虫感染性幼虫免疫小鼠后产生了几种单克隆抗体。其中一种在马来布鲁线虫微丝蚴表面产生阳性荧光反应，这种特定的单克隆抗体（IgM 同型）能够介导小鼠腹腔巨噬细胞在体外黏附并杀死马来布鲁线虫微丝蚴。新鲜的正常小鼠血清可增强黏附和杀伤作用，提示补体发挥了作用。当将相同的单克隆抗体被动转移至血液循环中有微丝蚴的小鼠体内时，在 70%的动物中观察到微丝蚴被完全清除。通过放射免疫沉淀法，这种单克隆抗体能够识别存在于马来布鲁线虫微丝蚴表面的（分子量为 110,000 的）抗原决定簇。
