使用二氢卟吩e6光敏剂进行光动力治疗期间的肿瘤荧光和氧合监测。
Tumor fluorescence and oxygenation monitoring during photodynamic therapy with chlorin e6 photosensitizer.
作者信息
Efendiev Kanamat, Alekseeva Polina, Linkov Kirill, Shiryaev Artem, Pisareva Tatiana, Gilyadova Aida, Reshetov Igor, Voitova Arina, Loschenov Victor
机构信息
Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia; National Research Nuclear University "MEPhI", Moscow, Russia.
Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia.
出版信息
Photodiagnosis Photodyn Ther. 2024 Feb;45:103969. doi: 10.1016/j.pdpdt.2024.103969. Epub 2024 Jan 10.
BACKGROUND
The study is aimed at developing a method for monitoring photodynamic therapy (PDT) of a tumor using chlorin-type photosensitizers (PSs). Lack of monitoring of chlorin e6 (Cе6) photobleaching, hemoglobin oxygenation and blood flow during light exposure can limit the PDT effectiveness.
MATERIALS AND METHODS
Phototheranostics includes spectral-fluorescence diagnostics of Ce6 distribution in the NIR range and PDT with simultaneous assessment of hemoglobin oxygenation and tumor blood flow. Fluorescence diagnostics and PDT were performed using the single laser λ=660 ± 5 nm.
RESULTS
Combined spectroscopic PDT monitoring method allowed simultaneous estimation of Ce6 photobleaching, hemoglobin oxygenation and tumor vascular thrombosis during PDT without interrupting the therapeutic light exposure.
CONCLUSION
The developed method of tumor phototheranostics using chlorin-type PSs may make it possible to personalize the duration of therapeutic light exposure during PDT.
背景
本研究旨在开发一种使用二氢卟吩型光敏剂（PSs）监测肿瘤光动力疗法（PDT）的方法。在光照期间缺乏对二氢卟吩e6（Ce6）光漂白、血红蛋白氧合和血流的监测可能会限制PDT的有效性。
材料与方法
光诊疗包括在近红外范围内对Ce6分布进行光谱荧光诊断以及在评估血红蛋白氧合和肿瘤血流的同时进行PDT。使用单一激光λ=660±5nm进行荧光诊断和PDT。
结果
联合光谱PDT监测方法能够在不中断治疗性光照的情况下，在PDT期间同时估计Ce6光漂白、血红蛋白氧合和肿瘤血管血栓形成情况。
结论
所开发的使用二氢卟吩型PSs的肿瘤光诊疗方法可能使个性化PDT期间治疗性光照的持续时间成为可能。
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