State Key Laboratory of High Performance Ceramics, and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, P. R. China.
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Angew Chem Int Ed Engl. 2020 Jan 27;59(5):1906-1913. doi: 10.1002/anie.201912824. Epub 2019 Dec 13.
Sustained tumor oxygenation is of critical importance during type-II photodynamic therapy (PDT), which depends on the intratumoral oxygen level for the generation of reactive oxygen species. Herein, the modification of photosynthetic cyanobacteria with the photosensitizer chlorin e6 (ce6) to form ce6-integrated photosensitive cells, termed ceCyan, is reported. Upon 660 nm laser irradiation, sustained photosynthetic O evolution by the cyanobacteria and the immediate generation of reactive singlet oxygen species ( O ) by the integrated photosensitizer could be almost simultaneously achieved for tumor therapy using type-II PDT both in vitro and in vivo. This work contributes a conceptual while practical paradigm for biocompatible and effective PDT using hybrid microorganisms, displaying a bright future in clinical PDT by microbiotic nanomedicine.
在 II 型光动力疗法（PDT）中，肿瘤内的氧气水平对于产生活性氧至关重要。本文报道了用光敏剂氯乙酮（Ce6）对光合蓝细菌进行修饰，形成 Ce6 整合的光敏细胞，称为 CeCyan。在 660nm 激光照射下，体外和体内肿瘤治疗均可同时实现蓝细菌持续的光合 O2 释放和整合光敏剂即刻产生活性单线态氧（1O2），用于 II 型 PDT。这项工作为使用混合微生物进行生物相容性和有效的 PDT 提供了一个概念性的实用范例，通过微生物纳米医学在临床 PDT 中展现了光明的前景。
