一种估算血液中多萜醇的微量方法。

A micromethod for the estimation of blood dolichol.

作者信息

Morris G N, Pullarkat R K

出版信息

Lipids. 1987 Jan;22(1):58-60. doi: 10.1007/BF02534876.

Abstract

A micromethod for the estimation of dolichol in blood was developed using high performance liquid chromatography. This method can be applied to whole blood or plasma. With detector sensitivity set at 0.005 Absorbance Unit Full Scale, samples as little as 50 microliter are sufficient to carry out the determination. Blood samples from the tail vein of inbred strains of C57 BL/6 NNia-1 mice 3, 6, 12 and 24 mo of age were examined. Blood dolichol levels decreased with age from 163.3 ng/ml at 6 mo to 110.1 ng/ml at 24 mo. The major dolichol homologs were C-85 (10.4%), C-90 (41.6%), C-95 (38.0%) and C-100 (8.1%).

摘要

采用高效液相色谱法建立了一种测定血液中多萜醇的微量方法。该方法可应用于全血或血浆。将检测器灵敏度设置为0.005吸光度单位满量程时，仅50微升的样品就足以进行测定。对3、6、12和24月龄的近交系C57 BL/6 NNia-1小鼠尾静脉采集的血样进行了检测。血液中多萜醇水平随年龄增长而下降，从6月龄时的163.3纳克/毫升降至24月龄时的110.1纳克/毫升。主要的多萜醇同系物为C-85（10.4%）、C-90（41.6%）、C-95（38.0%）和C-100（8.1%）。