用于测定组织和血浆中多萜醇的高效液相色谱法。
High-performance liquid chromatographic method for the determination of dolichols in tissues and plasma.
作者信息
Yamada K, Yokohama H, Abe S, Katayama K, Sato T
出版信息
Anal Biochem. 1985 Oct;150(1):26-31. doi: 10.1016/0003-2697(85)90437-3.
High-performance liquid chromatographic methods for the determination of dolichols in tissues and plasma have been developed. The tissue concentration of dolichols was measured by high-performance liquid chromatography with uv detection and plasma levels of dolichols were determined fluorometrically after derivatization with anthracene-9-carboxylic acid. In both methods, 2,2-didecaprenylethanol was used as an internal standard. The method with fluorescence detection was sufficiently sensitive to measure the concentration of dolichols in human plasma.
已开发出用于测定组织和血浆中多萜醇的高效液相色谱法。通过带紫外检测的高效液相色谱法测量多萜醇的组织浓度，并用9-蒽甲酸衍生化后通过荧光法测定多萜醇的血浆水平。在这两种方法中，2,2-二十二异戊烯基乙醇用作内标。荧光检测法灵敏度足够高，能够测量人血浆中多萜醇的浓度。
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