Onwusereaka Cynthia Oluchi, Jalaludin Juliana, Oluchi Sampson Emilia, Poh Choo Veronica Chua
Department of Environmental and Occupational Health, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Serdang, Selangor, Malaysia.
Department of Community Health, Faculty of Medicine and Health Science, Universiti Putra Malaysia Serdang, Selangor, Malaysia.
Rev Environ Health. 2024 Jan 15;40(1):47-62. doi: 10.1515/reveh-2023-0004. Print 2025 Mar 26.
The exposure of occupants to indoor air pollutants has increased in recent decades. The aim of this review is to discuss an overview of new approaches that are used to study fungal aerosols. Thus, this motivation was to compensate the gaps caused by the use of only traditional approaches in the study of fungal exposure.
The search involved various databases such as; Science Direct, PubMed, SAGE, Springer Link, EBCOHOST, MEDLINE, CINAHL, Cochrane library, Web of Science and Wiley Online Library. It was limited to full text research articles that reported the use of non-viable method in assessing bioaerosol, written in English Language, full text publications and published from year 2015-2022.
A total of 15 articles met the inclusion criteria and was included in this review. The use of next-generation sequencing, which is more commonly referred to as high-throughput sequencing (HTS) or molecular methods in microbial studies is based on the detection of genetic material of organisms present in a given sample. Applying these methods to different environments permitted the identification of the microorganisms present, and a better comprehension of the environmental impacts and ecological roles of microbial communities. Based on the reviewed articles, there is evidence that dust samples harbour a high diversity of human-associated bacteria and fungi. Molecular methods such as next generation sequencing are reliable tools for identifying and tracking the bacterial and fungal diversity in dust samples using 18S metagenomics approach.
近几十年来，居住者接触室内空气污染物的情况有所增加。本综述的目的是讨论用于研究真菌气溶胶的新方法概述。因此，这样做的动机是弥补在真菌暴露研究中仅使用传统方法所造成的差距。
检索涉及多个数据库，如科学Direct、PubMed、SAGE、Springer Link、EBCOHOST、MEDLINE、CINAHL、Cochrane图书馆、科学网和Wiley Online Library。检索限于2015年至2022年期间以英文撰写的、报道了使用非活性方法评估生物气溶胶的全文研究文章和全文出版物。
共有15篇文章符合纳入标准并被纳入本综述。在微生物研究中，更常被称为高通量测序（HTS）或分子方法的下一代测序基于检测给定样本中存在的生物体的遗传物质。将这些方法应用于不同环境可以识别存在的微生物，并更好地理解微生物群落的环境影响和生态作用。根据所综述的文章，有证据表明灰尘样本中含有高度多样的与人类相关的细菌和真菌。下一代测序等分子方法是使用18S宏基因组学方法识别和追踪灰尘样本中细菌和真菌多样性的可靠工具。
