Glick B S, Rothman J E
Nature. 1987;326(6110):309-12. doi: 10.1038/326309a0.
The transport of proteins between subcellular compartments is a vectorial, energy-requiring process mediated by the budding and fusion of a series of vesicular carriers. As yet, nothing is known of the chemical reactions that underlie these events, or how or in exactly what forms energy is used to sustain such movements. Here we report that fatty acyl-CoA acts as cofactor to a Golgi-associated protein factor (termed NSF) that is required for transport between cisternae of the Golgi stack in a cell-free system. This previously unsuspected connection may offer a link between the complex process of protein transport and a single, well-defined type of chemical reaction. We suggest that an ATP-dependent cycle of fatty acylation and deacylation may play an important role in driving rounds of vectorial protein transport.
蛋白质在亚细胞区室之间的运输是一个由一系列囊泡载体的出芽和融合介导的、需要能量的定向过程。迄今为止，对于这些事件背后的化学反应，或者能量如何以及以何种确切形式被用于维持此类运输，我们仍一无所知。在此，我们报告脂肪酰辅酶A作为一种与高尔基体相关的蛋白质因子（称为NSF）的辅因子，该因子在无细胞系统中高尔基体堆叠的潴泡间运输中是必需的。这种先前未被怀疑的联系可能为蛋白质运输的复杂过程与一种单一的、明确的化学反应类型之间提供了一个纽带。我们认为，脂肪酰化和去酰化的ATP依赖循环可能在驱动一轮轮的定向蛋白质运输中发挥重要作用。
