Morimoto K, Masana Y, Hashimoto T, Jamshidi J, Hayakawa T, Ikeda T, Mogami H
No Shinkei Geka. 1987 Jan;15(1):25-30.
A prospective high performance liquid chromatography (HPLC) study was performed in eighteen patients with head injury and stroke and four control volunteers to evaluate creatinine and purine metabolites concentration (adenosine, inosine, hypoxanthine, uric acid) in cerebrospinal fluid (CSF). The present HPLC method is rapid, accurate and sensitive in the same isocratic run and no specimen pretreatment of 0.02 ml CSF is necessary. The creatinine level in CSF was increased from 122 to 169 mumol/l in some patients, and was found unrelated to that of serum. The uric acid levels varied between 5.8 and 121 mumol/l and were associated with decrease in Glasgow Coma Scale score and had a critical point of 30 mumol/l. We present initial results in application of HPLC method to measure the creatinine and purine metabolites in CSF. This preliminary report presents that these high levels in CSF of head injury and stroke patients probably reflect tissue damage and an increased tissue catabolism.
对18例头部受伤和中风患者以及4名对照志愿者进行了一项前瞻性高效液相色谱（HPLC）研究，以评估脑脊液（CSF）中肌酐和嘌呤代谢物浓度（腺苷、肌苷、次黄嘌呤、尿酸）。当前的HPLC方法在相同的等度运行中快速、准确且灵敏，无需对0.02 ml脑脊液进行标本预处理。部分患者脑脊液中的肌酐水平从122 μmol/L升至169 μmol/L，且发现与血清肌酐水平无关。尿酸水平在5.8至121 μmol/L之间变化，与格拉斯哥昏迷量表评分降低相关，临界点为30 μmol/L。我们展示了应用HPLC方法测量脑脊液中肌酐和嘌呤代谢物的初步结果。这份初步报告表明，头部受伤和中风患者脑脊液中的这些高水平可能反映了组织损伤和组织分解代谢增加。
