Bao Li, Wu Bowen, Yang Chunjing, Xu Xiqiao, Shi Zhengyuan, Jiang Dechun
Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China.
Beijing Key Laboratory of Bio-Characteristic Profiling for Evaluation of Rational Drug Use, Beijing, People's Republic of China.
Diabetes Metab Syndr Obes. 2024 Jan 10;17:203-212. doi: 10.2147/DMSO.S444987. eCollection 2024.
Amino acids are the important metabolites in the body and play a crucial role in biological processes. The purpose of this study is to provide a profile of amino acids change in the serum of T2DM patients and identify potential biomarkers.
In this study, we quantitatively determined the serum amino acid profiles of 30 T2DM patients and 30 healthy volunteers. test and multivariate statistical analysis were used to identify candidate biomarkers with GraphPad Prism 9.5 software and MetaboAnalyst 5.0 on-line platform.
Thirty-four amino acids were quantified, and 19 amino acid levels differed significantly between T2DM and Healthy groups. Screened by the specific screening criteria (VIP>1.0; P<0.05; FC>1.5, or FC<0.67) in MetaboAnalyst 5.0 platform, 8 amino acids were identified as potential biomarkers. Pearson rank correlation test showed 14 differential amino acids were significantly correlated with T2DM-related physiological parameters.
The results of this study provide theoretical basis for the subsequent development of dietary supplements for the prevention or treatment of T2DM and its complications.
氨基酸是体内重要的代谢产物，在生物过程中起着关键作用。本研究的目的是提供2型糖尿病（T2DM）患者血清中氨基酸变化的概况，并确定潜在的生物标志物。
在本研究中，我们定量测定了30例T2DM患者和30例健康志愿者的血清氨基酸谱。使用GraphPad Prism 9.5软件和MetaboAnalyst 5.0在线平台进行检验和多变量统计分析，以识别候选生物标志物。
共定量了34种氨基酸，T2DM组和健康组之间有19种氨基酸水平存在显著差异。在MetaboAnalyst 5.0平台上按照特定筛选标准（VIP>1.0；P<0.05；FC>1.5或FC<0.67）进行筛选，确定了8种氨基酸为潜在生物标志物。Pearson秩相关检验显示，14种差异氨基酸与T2DM相关生理参数显著相关。
本研究结果为后续开发用于预防或治疗T2DM及其并发症的膳食补充剂提供了理论依据。
