Yadav Pradeep Kumar, Kumar Arun, Pandey Prashasti, Kumar Deepak, Singh Anita
Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, UP 221005 India.
Physiol Mol Biol Plants. 2023 Dec;29(12):2021-2033. doi: 10.1007/s12298-023-01389-3. Epub 2023 Dec 24.
The present study is based on the application of HS as an exogenous antidote in (spinach) plants grown in Cd-contaminated (50 ppm) soil. The different doses of HS in the form of NaHS (10, 50, 100, 200, and 500 μM) have been applied as a foliar spray to regulate the physiological attributes under Cd toxicity. Over to control, the plants grown in Cd alone showed a reduction in the fresh biomass by 48% with more production of oxidative biomarkers (HO, SOR, and MDA content) and antioxidative enzymes (SOD, POD, APX, and GR). Further, with the exogenous application of HS, among all the doses the fresh biomass was found to be maximally increased at 100 μM dose by 76%, and the Cd content was reduced significantly by 25% in the shoot compared to plants grown in Cd treated soil alone. With the decrease in Cd content in the shoot, the production of HO, SOR, and MDA content was reduced by 52%, 40%, and 38% respectively, at 100 μM compared to the plants grown in Cd-treated soil. The activities of estimated antioxidative enzymes showed a reduction in their activities up to 100 μM. Whereas, Glutathione reductase (GR) and Phytochelatins (PCs) showed different trends with their higher values in plants treated with NaHS in the presence of Cd. At 100 μM the GR and PCs, respectively showed 48% and 37% increment over Cd-treated plants alone. At this dose, the relative expression of , , , , and (Phytochelatin synthetase enzyme) genes, and other functional activities (SEM and fluorescence kinetics) supported the best performance of plants at 100 μM. Therefore, among all the doses, 100 μM dose of HS has significantly reduced the Cd toxicity by maintaining the growth and other functional traits of plants. The correlation analysis also supported the result by showing a relationship between HS application and Cd uptake. So, with this strategy, the plants grown in metal-contaminated fields can be improved qualitatively as well as quantitatively. With further experimentation, the mode of application could be explored to increase its efficiency and to promote this strategy at a wider scale.
The online version contains supplementary material available at 10.1007/s12298-023-01389-3.
本研究基于在受镉污染（50 ppm）土壤中生长的（菠菜）植株上应用硫化氢（HS）作为外源解毒剂。以硫氢化钠（NaHS）形式的不同剂量的HS（10、50、100、200和500 μM）已作为叶面喷雾剂施用，以调节镉毒性下的生理特性。与对照相比，仅在镉中生长的植株鲜生物量减少了48%，氧化生物标志物（过氧化氢（HO）、超氧化物歧化酶（SOR）和丙二醛（MDA）含量）和抗氧化酶（超氧化物歧化酶（SOD）、过氧化物酶（POD）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR））的产量增加。此外，通过外源施用HS，在所有剂量中，发现100 μM剂量时鲜生物量最大增加了76%，与仅在镉处理土壤中生长的植株相比，地上部镉含量显著降低了25%。随着地上部镉含量的降低，与在镉处理土壤中生长的植株相比，100 μM时HO、SOR和MDA含量分别降低了52%、40%和38%。估计的抗氧化酶活性在100 μM之前其活性降低。然而，谷胱甘肽还原酶（GR）和植物螯合肽（PCs）表现出不同的趋势，在镉存在下用NaHS处理的植株中它们的值更高。在100 μM时，GR和PCs分别比仅镉处理的植株增加了48%和37%。在此剂量下，相关基因（植物螯合肽合成酶基因）以及其他功能活性（扫描电子显微镜（SEM）和荧光动力学）的相对表达支持了植株在100 μM时的最佳表现。因此，在所有剂量中，100 μM剂量的HS通过维持植株的生长和其他功能特性显著降低了镉毒性。相关性分析也通过显示HS施用与镉吸收之间的关系支持了该结果。所以，通过这种策略，在金属污染田地中生长的植株在质量和数量上都可以得到改善。通过进一步实验，可以探索施用方式以提高其效率并在更广泛的范围内推广该策略。
在线版本包含可在10.1007/s12298-023-01389-3获取的补充材料。
