Department of Precision Medicine, University of Campania "Luigi Vanvitelli", via L. De Crecchio 7, 80138 Naples, Italy.
Multidisciplinary Department of Medical, Surgical and Dental Specialties, University of Campania "Luigi Vanvitelli", 80138, Naples, Italy.
Int J Mol Sci. 2019 Mar 6;20(5):1153. doi: 10.3390/ijms20051153.
The role of sirtuin 6 (SIRT6) in adipose abdominal tissue of pre-diabetic (pre-DM) patients is poorly known. Here, we evaluated SIRT6 expression in visceral abdominal fat of obese pre-diabetic patients and the potential effects of metformin therapy. Results indicated that obese pre-DM subjects showed low SIRT6 protein expression and high expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), peroxisome proliferator-activated receptor gamma (PPAR-γ), and sterol regulatory element-binding transcription factor 1 (SREBP-1). Obese pre-DM patients showed high values of glucose, insulin resistance (HOMA-IR), C reactive protein (CRP), nitrotyrosine, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6), and low values of insulin ( < 0.05). Of note, abdominal fat tissue of obese pre-DM patients treated with metformin therapy presented higher SIRT6 expression and lower NF-κB, PPAR-γ, and SREBP-1 expression levels compared to pre-DM control group. Collectively, results show that SIRT6 is involved in the inflammatory pathway of subcutaneous abdominal fat of obese pre-DM patients and its expression responds to metformin therapy.
Sirtuin 6（SIRT6）在糖尿病前期（pre-DM）患者腹部脂肪组织中的作用尚不清楚。在这里，我们评估了肥胖 pre-DM 患者内脏腹部脂肪中的 SIRT6 表达情况，以及二甲双胍治疗的潜在影响。结果表明，肥胖的 pre-DM 患者表现出低 SIRT6 蛋白表达和高水平的核因子 kappa-轻链增强子的 B 细胞（NF-κB）、过氧化物酶体增殖物激活受体 γ（PPAR-γ）和固醇调节元件结合转录因子 1（SREBP-1）。肥胖的 pre-DM 患者表现出高血糖、胰岛素抵抗（HOMA-IR）、C 反应蛋白（CRP）、硝基酪氨酸、肿瘤坏死因子-α（TNF-α）和白细胞介素 6（IL-6），以及胰岛素水平低（<0.05）。值得注意的是，与 pre-DM 对照组相比，接受二甲双胍治疗的肥胖 pre-DM 患者的腹部脂肪组织中 SIRT6 表达水平较高，而 NF-κB、PPAR-γ 和 SREBP-1 的表达水平较低。综上所述，结果表明 SIRT6 参与了肥胖 pre-DM 患者皮下腹部脂肪的炎症途径，其表达对二甲双胍治疗有反应。
