Chen Le Min, Keisham Sunanda, Tateno Hiroaki, van Ede Jitske, Pronk Mario, van Loosdrecht Mark C M, Lin Yuemei
Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
ACS ES T Water. 2023 Dec 27;4(1):279-286. doi: 10.1021/acsestwater.3c00625. eCollection 2024 Jan 12.
Bacteria can synthesize a diverse array of glycans, being found attached to proteins and lipids or as loosely associated polysaccharides to the cells. The major challenge in glycan analysis in environmental samples lies in developing high-throughput and comprehensive characterization methodologies to elucidate the structure and monitor the change of the glycan profile, especially in protein glycosylation. To this end, in the current research, the dynamic change of the glycan profile of a few extracellular polymeric substance (EPS) samples was investigated by high-throughput lectin microarray and mass spectrometry, as well as sialylation and sulfation analysis. Those EPS were extracted from aerobic granular sludge collected at different stages during its adaptation to the seawater condition. It was found that there were glycoproteins in all of the EPS samples. In response to the exposure to seawater, the amount of glycoproteins and their glycan diversity displayed an increase during adaptation, followed by a decrease once the granules reached a stable state of adaptation. Information generated sheds light on the approaches to identify and monitor the diversity and dynamic alteration of the glycan profile of the EPS in response to environmental stimuli.
细菌能够合成各种各样的聚糖，这些聚糖可以附着在蛋白质和脂质上，或者以松散结合的多糖形式存在于细胞中。环境样品中聚糖分析的主要挑战在于开发高通量和全面的表征方法，以阐明聚糖结构并监测聚糖谱的变化，尤其是在蛋白质糖基化方面。为此，在当前的研究中，通过高通量凝集素微阵列和质谱以及唾液酸化和硫酸化分析，研究了一些胞外聚合物（EPS）样品聚糖谱的动态变化。这些EPS是从好氧颗粒污泥在适应海水条件的不同阶段收集的。结果发现，所有EPS样品中都存在糖蛋白。响应于海水暴露，在适应过程中糖蛋白的数量及其聚糖多样性呈现增加趋势，而一旦颗粒达到稳定的适应状态则随后减少。所产生的信息为识别和监测EPS聚糖谱响应环境刺激的多样性和动态变化的方法提供了线索。
