聚甲基丙烯酸甲酯-羟基磷灰石对口腔细菌的抗菌和抗真菌活性：一项体外研究。

Polymethylmethacrylate-hydroxyapatite antibacterial and antifungal activity against oral bacteria: An in vitro study.

作者信息

Saskianti Tania, Wardhani Karina K, Fadhila Naura, Wahluyo Soegeng, Dewi Ardianti M, Nugraha Alexander P, Ernawati Diah S, Kanawa Masami

机构信息

Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Indonesia.

Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Indonesia.

出版信息

J Taibah Univ Med Sci. 2023 Nov 21;19(1):190-197. doi: 10.1016/j.jtumed.2023.11.001. eCollection 2024 Feb.

DOI:10.1016/j.jtumed.2023.11.001

PMID:38229827

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10790095/

Abstract

OBJECTIVE

Reconstruction of alveolar bone defects resulting from aging, trauma, ablative surgery or pathology, remains a significant clinical challenge. The objective of this study was to investigate the antibacterial and antifungal activities of mixed polymethylmethacrylate-hydroxyapatite (PMMA-HA) against oral microorganisms. Our findings could provide valuable insights into the prospective application of PMMA-HA as a synthetic bone graft material to manage alveolar bone defects tissue engineering.

METHODS

HA powder was obtained from the Center for Ceramics in Indonesia and PMMA granules were obtained from HiMedia Laboratories; these were prepared in 20:80, 30:70, and 40:60 ratios. The antibacterial diffusion method was then performed against , , , and , while the antifungal diffusion method was used to test against . Standardized protocols were used for microbial culturing and inhibition zones were measured with digital calipers. Statistical analyses included one-way ANOVA and Kruskal-Wallis tests, supplemented by post-hoc Tukey HSD tests.

RESULTS

A PMMA-HA scaffold with a 20:80 ratio demonstrated the highest antibacterial activity against , , , and . This was followed by the 30:70 and 40:60 ratios in terms of antibacterial activity. Statistical significance was achieved with p < 0.05 in comparison to controls. However, none of the PMMA-HA ratios showed antifungal activity against .

CONCLUSION

PMMA-HA scaffolds have significant activity against bacteria, but not against fungi.

摘要

目的

修复因衰老、创伤、切除手术或病理导致的牙槽骨缺损，仍然是一项重大的临床挑战。本研究的目的是调查聚甲基丙烯酸甲酯 - 羟基磷灰石（PMMA - HA）混合物对口腔微生物的抗菌和抗真菌活性。我们的研究结果可为PMMA - HA作为合成骨移植材料在牙槽骨缺损组织工程中的应用前景提供有价值的见解。

方法

HA粉末购自印度尼西亚陶瓷中心，PMMA颗粒购自HiMedia实验室；将它们按20:80、30:70和40:60的比例制备。然后采用抗菌扩散法检测对[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]和[具体细菌名称4]的抗菌活性，同时采用抗真菌扩散法检测对[具体真菌名称]的抗真菌活性。使用标准化方案进行微生物培养，并用数字卡尺测量抑菌圈。统计分析包括单因素方差分析和Kruskal - Wallis检验，并辅以事后Tukey HSD检验。

结果

比例为20:80的PMMA - HA支架对[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]和[具体细菌名称4]表现出最高的抗菌活性。其次是抗菌活性方面的30:70和40:60比例。与对照组相比，p < 0.05具有统计学意义。然而，没有一个PMMA - HA比例显示出对[具体真菌名称]的抗真菌活性。