Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy, Future University in Egypt, Cairo, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Braz J Microbiol. 2024 Mar;55(1):515-527. doi: 10.1007/s42770-023-01224-7. Epub 2024 Jan 17.
The surge in multidrug-resistant pathogens worldwide has jeopardized the clinical efficiency of many current antibiotics. This problem steered many researchers in their quest to discover new effective antimicrobial agents from natural origins including plants or their residing endophytes. In this work, we aimed to identify the endophytic fungi derived from Hedera helix L. and investigate their potential antimicrobial activity. Bioguided fractionation approach was conducted to isolate the pure compounds from the most active fungal fraction. Out of a total of six different isolated endophytic fungal strains, only Aspergillus cejpii showed the highest activity against all tested microbial strains. The most active fraction was the dichloromethane/methanol fraction (DCM:MeOH), where it showed significant activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Serratia marcescens, Acinetobacter baumannii, Salmonella typhi, and three drug-resistant clinical isolate strains including Methicillin-resistant Staphylococcus aureus (MRSA, H1), Pseudomonas aeruginosa (PS 16), and Acinetobacter baumannii (ACT 322) using tetracyline and kanamycin as the control antibiotics. Bioguided fractionation of the active fraction led to the isolation of the γ-butenolide, spiculisporic acid. Structure elucidation was carried out using H and C-NMR spectroscopic analysis. The compound showed good antimicrobial activities with minimum inhibitory concentration (MIC) values ranging from 3.9 to 31.25 μg/mL against all tested strains. Gas chromatography coupled to mass spectrometry (GC-MS) profiling was also carried out to identify the metabolites in the microbial crude extract. In conclusion, endophytic fungi, Aspergillus cejpii, isolated from Hedera helix L. roots showed promising antimicrobial activity which merits further in-depth investigations for potential utilization as a source of new antibiotics in the future. It can also be considered as a novel source for spiculisporic acid.
全球范围内多药耐药病原体的激增危及了许多当前抗生素的临床疗效。这一问题促使许多研究人员从植物或其内生真菌等天然来源中寻找新的有效抗菌剂。在这项工作中，我们旨在鉴定来自常春藤的内生真菌，并研究它们的潜在抗菌活性。采用生物导向分离方法从最具活性的真菌部分中分离出纯化合物。在总共分离出的六种不同内生真菌菌株中，只有 Aspergillus cejpii 对所有测试的微生物菌株表现出最高的活性。最活跃的部分是二氯甲烷/甲醇部分（DCM:MeOH），它对大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌、粘质沙雷氏菌、鲍曼不动杆菌、伤寒沙门氏菌和三种耐多药临床分离株表现出显著的活性，包括耐甲氧西林金黄色葡萄球菌（MRSA，H1）、铜绿假单胞菌（PS 16）和鲍曼不动杆菌（ACT 322），四环素和卡那霉素作为对照抗生素。活性部分的生物导向分离导致 γ-丁烯内酯、spiculisporic acid 的分离。通过 H 和 C-NMR 光谱分析进行结构阐明。该化合物表现出良好的抗菌活性，对所有测试菌株的最小抑菌浓度（MIC）值在 3.9 至 31.25 μg/mL 之间。还进行了气相色谱-质谱联用（GC-MS）分析以鉴定微生物粗提物中的代谢物。总之，从常春藤根部分离出的内生真菌 Aspergillus cejpii 表现出有希望的抗菌活性，值得进一步深入研究，以作为未来新型抗生素的潜在来源。它也可以被认为是 spiculisporic acid 的一个新来源。
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