Techaoei Surachai, Jirayuthcharoenkul Chariya, Jarmkom Khemjira, Dumrongphuttidecha Thisakorn, Khobjai Warachate
Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi 12130 Thailand.
Innovation of Plants Extraction and Biological Activity for Pharmaceutical Products, Food Supplement and Medical Drugs, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi 12130 Thailand.
Saudi J Biol Sci. 2020 Nov;27(11):2883-2889. doi: 10.1016/j.sjbs.2020.08.037. Epub 2020 Sep 2.
The objective of this study was to characterize an endophytic fungi producing-bioactive compound from the aquatic plant, . All parts of such plant were cleaned with surface sterilization technique and cultured on potato dextrose agar to isolate endophytic fungi. The identification was characterized by morphological and molecular technique. Fungal isolates were screened to discover antimicrobial activities by disc diffusion method against Methicillin-resistant Staphylococcus aureus DMST20651 (MRSA). MIC and MBC for those crude fungal extracts were determined. Finally, the chemical profile of crude extract was determined by gas chromatography and mass spectrometry. Six endophytic fungi were isolated from the surface-satirized parts of . Based on disc diffusion assay, the highest antibacterial activity against MRSA was isolate ST9.1 identified as . Results demonstrated that the ethyl acetate extraction had more active fractions with MIC of 2.5 mg/ml and MBC concentration of 50.0 mg/ml. The crude extracts were developed to identify the chemical constituents by gas chromatography and mass spectrometry. The major component of crude extract of endophytic fungi was 5-(1H-Indol-3-yl)-4,5-dihydro-[1,2,4]triazin-3-ylamine (CHN). Thus, the plant could be used in the treatment of infectious diseases caused by bacterial pathogen.
本研究的目的是鉴定一种从水生植物中产生生物活性化合物的内生真菌。该植物的所有部分均采用表面消毒技术进行清洗，并在马铃薯葡萄糖琼脂培养基上培养以分离内生真菌。通过形态学和分子技术对其进行鉴定。采用纸片扩散法，对分离得到的真菌菌株进行抗甲氧西林耐药金黄色葡萄球菌DMST20651（MRSA）抗菌活性筛选。测定了这些真菌粗提物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。最后，通过气相色谱和质谱法测定粗提物的化学组成。从经表面消毒的部分分离出6株内生真菌。基于纸片扩散试验，对MRSA抗菌活性最高的菌株是鉴定为[具体名称未给出]的ST9.1。结果表明，乙酸乙酯提取物具有更多活性成分，其MIC为2.5mg/ml，MBC浓度为50.0mg/ml。通过气相色谱和质谱法对粗提物进行分析以鉴定化学成分。内生真菌粗提物的主要成分是5-（1H-吲哚-3-基）-4,5-二氢-[1,2,4]三嗪-3-胺（CHN）。因此，该植物可用于治疗由细菌病原体引起的传染病。
