Hebei Key Laboratory of Infertility and Genetics, Hebei Clinical Research Center for Birth Defects, Hebei Medical Key Discipline of Reproductive Medicine, Hebei Collaborative Innovation Center of Integrated Traditional and Western Medicine on Reproductive Disease, Department of Reproductive Medicine, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, China.
Cardiovascular platform, Institute of Health and Disease, Hebei Medical University, Shijiazhuang, 050000, China.
BMC Med Genomics. 2024 Jan 19;17(1):26. doi: 10.1186/s12920-024-01806-w.
To compare the expression levels of long non-coding RNA (lncRNA) and messenger RNA (mRNA) in pre-receptive endometrium between patients with Polycystic Ovary Syndrome (PCOS)and normal ovulation undergoing in vitro fertilization-embryo transfer (IVF-ET).
Endometrial tissues were collected with endometrial vacuum curette in pre-receptive phase (3 days after oocytes retrieval) from PCOS and control groups. LncRNAs and mRNAs of endometrium were identified via RNA sequencing and alignments. A subset of 9 differentially expressed lncRNAs and 11 mRNAs were validated by quantitative reverse transcription polymerase chain reaction(qRT-PCR)in 22 PCOS patients and 18 ovulation patients. The function of mRNAs with differential expression patterns were explored using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).
We found out 687 up-regulated and 680 down-regulated mRNAs, as well as 345 up-regulated and 63 down-regulated lncRNAs in the PCOS patients in contrast to normal ovulation patients. qRT-PCR was used to detect the expression of 11 mRNAs, and validated that the expression of these 6 mRNAs CXCR4, RABL6, OPN3, SYBU, IDH1, NOP10 were significantly elevated among PCOS patients, and the expression of ZEB1 was significantly decreased. qRT-PCR was performed to detect the expression of 9 lncRNAs, and validated that the expression of these 7 lncRNAs IDH1-AS1, PCAT14, FTX, DANCR, PRKCQ-AS1, SNHG8, TPT1-AS1 were significantly enhanced among PCOS patients. Bioinformatics analysis showed that differentially expressed genes (DEGs) involved KEGG pathway were tyrosine metabolism, PI3K-Akt pathway, metabolic pathway, Jak-STAT pathway, pyruvate metabolism, protein processing in endoplasmic reticulum, oxidative phosphorylation and proteasome. The up-regulation of GO classification was involved in ATP metabolic process, oxidative phosphorylation, RNA catabolic process, and down-regulation of GO classification was response to corticosteroid, steroid hormone, and T cell activation.
Our results determined the characteristics and expression profile of endometrial lncRNAs and mRNAs in PCOS patients in pre-receptive phase, which is the day 3 after oocytes retrival. The possible pathways and related genes of endometrial receptivity disorders were found, and those lncRNAs may be developed as a predictive biomarker of endometrium in pre-receptive phase.
比较体外受精-胚胎移植（IVF-ET）过程中接受治疗的多囊卵巢综合征（PCOS）患者和正常排卵患者的着床前子宫内膜中长链非编码 RNA（lncRNA）和信使 RNA（mRNA）的表达水平。
在接受治疗的前阶段（取卵后 3 天），使用子宫内膜真空吸引器从 PCOS 组和对照组中收集子宫内膜组织。通过 RNA 测序和比对鉴定子宫内膜中的 lncRNA 和 mRNA。通过定量逆转录聚合酶链反应（qRT-PCR）在 22 名 PCOS 患者和 18 名排卵患者中验证了 9 个差异表达的 lncRNA 和 11 个 mRNA。使用基因本体论（GO）和京都基因与基因组百科全书（KEGG）探索具有差异表达模式的 mRNA 的功能。
与正常排卵患者相比，我们发现 PCOS 患者中有 687 个上调和 680 个下调的 mRNA，以及 345 个上调和 63 个下调的 lncRNA。通过 qRT-PCR 检测了 11 个 mRNA 的表达，并验证了这些 6 个 mRNA（CXCR4、RABL6、OPN3、SYBU、IDH1、NOP10）在 PCOS 患者中的表达明显升高，而 ZEB1 的表达明显降低。通过 qRT-PCR 检测了 9 个 lncRNA 的表达，并验证了这些 7 个 lncRNA（IDH1-AS1、PCAT14、FTX、DANCR、PRKCQ-AS1、SNHG8、TPT1-AS1）在 PCOS 患者中的表达明显升高。生物信息学分析表明，差异表达基因（DEGs）参与了 KEGG 途径，包括酪氨酸代谢、PI3K-Akt 途径、代谢途径、Jak-STAT 途径、丙酮酸代谢、内质网蛋白质加工、氧化磷酸化和蛋白酶体。GO 分类的上调涉及 ATP 代谢过程、氧化磷酸化、RNA 分解代谢过程，而下调涉及对皮质类固醇、甾体激素和 T 细胞激活的反应。
我们的研究结果确定了着床前阶段（取卵后 3 天）接受治疗的 PCOS 患者子宫内膜中 lncRNA 和 mRNA 的特征和表达谱。发现了子宫内膜容受性障碍的可能途径和相关基因，这些 lncRNA 可能被开发为着床前子宫内膜的预测生物标志物。
