Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, 710069, Xi'an, China.
School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, China.
Talanta. 2024 May 1;271:125674. doi: 10.1016/j.talanta.2024.125674. Epub 2024 Jan 20.
Abnormal levels of dopamine (DA) and uric acid (UA) in the human body are valuable indicators for monitoring human health, as they are associated with certain diseases. Therefore, it is crucial to develop sensitive and simultaneous analytical techniques for DA and UA in diagnosing the related diseases. Herein, the Co- and Mo- doped carbon nanofibers (Co, Mo@CNFs) electrochemical biosensor was developed successfully for the sensitive and accurate simultaneous detection of DA and UA. A straightforward electrospinning technique followed by a carbonization process was employed for the synthesis of Co, Mo@CNFs, and the encapsulation of Co and Mo within CNFs served to not only prevent nanoparticle agglomeration, thus providing more active sites, but also to facilitate rapid electron transfer. By incorporating Co and Mo into CNFs, the electrocatalytic activity of the modified electrode was greatly improved due to the beneficial conductivity and synergistic effects of transition metals. This enhancement effectively addressed issues such as the overlapping anodic peaks that occur when DA and UA are oxidized concurrently. Due to the mentioned synergistic contributions, the modified Co, Mo@CNFs electrode (Co, Mo@CNFs/GCE) achieved remarkable sensitivity for the simultaneous detection of DA and UA, while also exhibiting strong anti-interference ability. The detection limits for DA and UA were 2.35 nmol L and 0.16 μmol L, respectively. We applied the developed Co, Mo@CNFs/GCE electrochemical biosensor to detect DA and UA in 50-fold diluted serum and urine samples. The results affirm the biosensor's reliability and precision. Moreover, the developed Co, Mo@CNFs/GCE biosensor demonstrated excellent performance in simultaneously detecting DA and UA, providing an efficient and dependable detection approach for clinical diagnosis and bioanalysis.
体内多巴胺（DA）和尿酸（UA）水平异常是监测人体健康的有价值指标，因为它们与某些疾病有关。因此，开发用于诊断相关疾病的 DA 和 UA 的灵敏且同时的分析技术至关重要。在此，成功开发了 Co 和 Mo 共掺杂碳纳米纤维（Co，Mo@CNFs）电化学生物传感器，用于灵敏和准确地同时检测 DA 和 UA。采用简单的静电纺丝技术和碳化过程合成 Co，Mo@CNFs，将 Co 和 Mo 封装在 CNFs 中不仅可以防止纳米颗粒团聚，从而提供更多的活性位点，而且还可以促进快速电子转移。通过将 Co 和 Mo 掺入 CNFs 中，由于过渡金属的有益导电性和协同效应，修饰电极的电催化活性大大提高。这种增强有效地解决了 DA 和 UA 同时氧化时出现的重叠阳极峰的问题。由于协同作用的贡献，修饰后的 Co，Mo@CNFs 电极（Co，Mo@CNFs/GCE）对 DA 和 UA 的同时检测具有出色的灵敏度，同时还具有很强的抗干扰能力。DA 和 UA 的检测限分别为 2.35 nmol L 和 0.16 μmol L。我们将开发的 Co，Mo@CNFs/GCE 电化学生物传感器应用于 50 倍稀释的血清和尿液样本中检测 DA 和 UA。结果证实了该生物传感器的可靠性和精度。此外，开发的 Co，Mo@CNFs/GCE 生物传感器在同时检测 DA 和 UA 方面表现出优异的性能，为临床诊断和生物分析提供了一种高效可靠的检测方法。
