Batantou Mabandza Degrâce, Colletin Edlyne, Dagot Christophe, Quétel Isaure, Breurec Sébastien, Guyomard-Rabenirina Stéphanie
Transmission, Reservoir and Diversity of Pathogens Unit, Pasteur Institute of Guadeloupe, 97110 Pointe-à-Pitre, France.
University of Limoges, INSERM, CHU Limoges, RESINFIT, U1092, 87000 Limoges, France.
Antibiotics (Basel). 2024 Jan 16;13(1):87. doi: 10.3390/antibiotics13010087.
Waterborne faecal contamination is a major public health concern. The main objectives of this study were to investigate faecal contamination and () antibiotic resistance in recreational fresh water from Guadeloupe and to characterise the microbiome and resistome composition in biofilms from submerged rocks. Significant faecal contamination was observed at 14 freshwater sites. predominated (62%), followed by (11%) and spp. (11%). Of 152 isolated, none produced extended-spectrum beta-lactamases (ESBLs), but 7% showed resistance to streptomycin and 4% to tetracycline. Biofilm resistome analysis revealed clinically significant antibiotic-resistance genes (ARGs), including those coding for resistance to sulfonamides (), carbapenems (), and third-generation cephalosporins (). Mobile genetic elements (MGEs) (, , ) linked to resistance to aminoglycosides, beta-lactams, tetracycline, as well as heavy metal resistance determinants (, , , ) conferring resistance to copper, silver, cadmium, and mercury were also detected. Diverse bacterial phyla were found in biofilm samples, of which Proteobacteria, Bacteroidetes, Planctonomycetes, and Cyanobacteria were predominant. Despite the frequent presence of exceeding regulatory standards, the low levels of antibiotic-resistant bacteria in freshwater and of ARGs and MGEs in associated biofilms suggest limited antibiotic resistance in Guadeloupean recreational waters.
水源性粪便污染是一个主要的公共卫生问题。本研究的主要目的是调查瓜德罗普岛休闲淡水中的粪便污染和（）抗生素耐药性，并对淹没岩石生物膜中的微生物组和耐药组组成进行表征。在14个淡水站点观察到显著的粪便污染。（）占主导地位（62%），其次是（）（11%）和（）属（11%）。在分离出的152株（）中，没有一株产生超广谱β-内酰胺酶（ESBLs），但7%对链霉素耐药，4%对四环素耐药。生物膜耐药组分析揭示了具有临床意义的抗生素耐药基因（ARGs），包括那些编码对磺胺类药物（）、碳青霉烯类药物（）和第三代头孢菌素（）耐药的基因。还检测到与对氨基糖苷类、β-内酰胺类、四环素耐药相关的移动遗传元件（MGEs）（、、），以及赋予对铜、银、镉和汞耐药性的重金属抗性决定簇（、、、）。在生物膜样本中发现了多种细菌门，其中变形菌门、拟杆菌门、浮霉菌门和蓝细菌门占主导地位。尽管（）的含量经常超过监管标准，但淡水中抗生素耐药菌的低水平以及相关生物膜中ARGs和MGEs的低水平表明瓜德罗普岛休闲水域的抗生素耐药性有限。
