Li Siwei, Ma Zhihao, Zhang Xinyi, Cai Yibo, Han Chenggui, Wu Xuehong
College of Plant Protection, China Agricultural University, Haidian District, Beijing 100193, China.
J Fungi (Basel). 2023 Dec 31;10(1):30. doi: 10.3390/jof10010030.
In the present study, sixteen novel RNA mycoviruses co-infecting a single strain of (strain D40) were identified and molecularly characterized using metatranscriptome sequencing combined with a method for rapid amplification of cDNA ends. The fungal strain was isolated from diseased seedlings of sugar beet with damping-off symptoms. Based on genome analysis and phylogenetic analysis of amino acid sequences of RNA-dependent RNA polymerase, the sixteen mycoviruses associated with strain D40 contained three genome types with nine distinct lineages, including positive single-stranded RNA (, , , and ), double-stranded RNA (Phlegiviridae, , Megatotiviridae, and Yadonushiviridae), and negative single-stranded RNA (), suggesting a complex composition of a mycoviral community in this single strain of (strain D40). Full genome sequences of six novel mycoviruses and the nearly full-length sequences of the remaining ten novel mycoviruses were obtained. Furthermore, seven of these sixteen mycoviruses were confirmed to assemble virus particles present in the strain D40. To the best of our knowledge, this is the first detailed study of mycoviruses infecting .
在本研究中，利用元转录组测序结合cDNA末端快速扩增方法，鉴定并分子表征了共感染单一菌株（菌株D40）的16种新型RNA真菌病毒。该真菌菌株从患有猝倒症状的甜菜病苗中分离得到。基于RNA依赖的RNA聚合酶氨基酸序列的基因组分析和系统发育分析，与菌株D40相关的16种真菌病毒包含三种基因组类型和九个不同的谱系，包括正单链RNA（，，，和）、双链RNA（分体负链RNA病毒科，，巨病毒科和亚多努什病毒科）以及负单链RNA（），这表明该单一菌株（菌株D40）中真菌病毒群落的组成复杂。获得了6种新型真菌病毒的全基因组序列以及其余10种新型真菌病毒的近全长序列。此外，这16种真菌病毒中的7种被证实可在菌株D40中组装病毒粒子。据我们所知，这是首次对感染的真菌病毒进行的详细研究。
