College of Plant Protection, China Agricultural University, Beijing 100193, China.
College of Horticulture, China Agricultural University, Beijing 100193, China.
Viruses. 2022 Apr 14;14(4):813. doi: 10.3390/v14040813.
Six novel mycoviruses that collectively represent the mycovirome of anastomosis group (AG)-3 PT strain ZJ-2H, which causes potato black scurf, were identified through metatranscriptome sequencing and putatively designated as Rhizoctonia solani fusarivirus 4 [RsFV4, positive single-stranded RNA (+ssRNA)], Rhizoctonia solani fusarivirus 5 (RsFV5, +ssRNA), Rhizoctonia solani mitovirus 40 (RsMV40, +ssRNA), Rhizoctonia solani partitivirus 10 [RsPV10, double-stranded RNA (dsRNA)], Rhizoctonia solani partitivirus 11 (RsPV11, dsRNA), and Rhizoctonia solani RNA virus 11 (RsRV11, dsRNA). Whole genome sequences of RsFV4, RsMV40, RsPV10, RsPV11, and RsRV11, as well as a partial genome sequence of RsFV5, were obtained. The 3'- and 5'- untranslated regions of the five mycoviruses with complete genome sequences were folded into stable stem-loop or panhandle secondary structures. RsFV4 and RsFV5 are most closely related to Rhizoctonia solani fusarivirus 1 (RsFV1), however, the first open reading frame (ORF) of RsFV4 and RsFV5 encode a hypothetical protein that differs from the first ORF of RsFV1, which encodes a helicase. We confirmed that RsPV10 and RsPV11 assemble into the spherical virus particles (approximately 30 nm in diameter) that were extracted from strain ZJ-2H. This is the first report that +ssRNA and dsRNA viruses co-infect a single strain of AG-3 PT.
通过宏转录组测序，共鉴定了 6 种新型真菌病毒，它们共同构成了引起马铃薯黑痣病的不亲和群（AG）-3PT 菌株 ZJ-2H 的真菌病毒组，分别被暂定命名为茄病镰刀菌丝状病毒 4（RsFV4，正单链 RNA(+ssRNA)）、茄病镰刀菌丝状病毒 5（RsFV5，+ssRNA）、茄病镰刀菌密旋病毒 40（RsMV40，+ssRNA）、茄病镰刀菌二分体病毒 10（RsPV10，双链 RNA(dsRNA)）、茄病镰刀菌二分体病毒 11（RsPV11，dsRNA）和茄病镰刀菌 RNA 病毒 11（RsRV11，dsRNA）。获得了 RsFV4、RsMV40、RsPV10、RsPV11 和 RsRV11 的全基因组序列，以及 RsFV5 的部分基因组序列。5 种具有完整基因组序列的真菌病毒的 3'-和 5'-非翻译区折叠成稳定的茎环或发夹二级结构。RsFV4 和 RsFV5 与茄病镰刀菌丝状病毒 1（RsFV1）最为密切相关，但 RsFV4 和 RsFV5 的第一个开放阅读框（ORF）编码的是一种假定蛋白，与 RsFV1 的第一个 ORF 编码的解旋酶不同。我们证实 RsPV10 和 RsPV11 组装成从菌株 ZJ-2H 中提取的球形病毒颗粒（直径约 30nm）。这是首次报道+ssRNA 和 dsRNA 病毒共同感染单个 AG-3PT 菌株。
