Liu Mingming, Liu Xintao, Zhao Hui, Ni Yunxia, Jia Min, Hu Peilin, Liu Hongyan, Tian Baoming
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Postgraduate T&R Base of Zhengzhou University, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Key Laboratory of Crop Pest Control, Zhengzhou, 450002, Henan, China.
School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Arch Virol. 2022 May;167(5):1375-1379. doi: 10.1007/s00705-022-05428-4. Epub 2022 Apr 9.
Corynespora cassiicola is an important phytopathogenic fungus that severely impairs crop production. Here, we report the molecular characterization of a novel positive-sense single-stranded RNA (+ssRNA) mycovirus, Corynespora cassiicola fusarivirus 1 (CcFV1), isolated from C. cassiicola strain 20200826-3-1. Excluding the poly(A) tail, the genome of the virus is 6491 nt in length and contains three putative open reading frames (ORFs). The large ORF1 encodes a polypeptide of 1524 aa with a conserved RNA-dependent RNA polymerase (RdRp) domain and a helicase (Hel) domain. BLASTp analysis showed that CcFV1 ORF1 has the highest similarity to Setosphaeria turcica fusarivirus 1 (StFV1, 50.45% identity, E-value 0.0). ORF2 encodes a polypeptide with a conserved chromosome segregation ATPase (Smc) domain. The smaller ORF3 encodes a polypeptide with an unknown function. Phylogenetic analysis based on the ORF1- encoded polypeptide showed that CcFV1 is phylogenetically related to members of the newly proposed family "Fusariviridae". Thus, we suggest that CcFV1 might be a novel member of the family "Fusariviridae", and is the first to be discovered in C. cassiicola.
橡胶棒孢炭疽菌是一种重要的植物病原真菌，严重损害作物产量。在此，我们报告了从橡胶棒孢炭疽菌菌株20200826 - 3 - 1中分离出的一种新型正义单链RNA（+ssRNA）真菌病毒——橡胶棒孢炭疽菌镰孢病毒1（CcFV1）的分子特征。除去poly(A)尾，该病毒基因组长度为6491 nt，包含三个推定的开放阅读框（ORF）。大的ORF1编码一个1524个氨基酸的多肽，具有保守的RNA依赖RNA聚合酶（RdRp）结构域和一个解旋酶（Hel）结构域。BLASTp分析表明，CcFV1的ORF1与玉米大斑病菌镰孢病毒1（StFV1）具有最高的相似性（同一性为50.45%，E值为0.0）。ORF2编码一个具有保守的染色体分离ATP酶（Smc）结构域的多肽。较小的ORF3编码一个功能未知的多肽。基于ORF1编码多肽的系统发育分析表明，CcFV1在系统发育上与新提出的“镰孢病毒科”家族成员相关。因此，我们认为CcFV1可能是“镰孢病毒科”家族的一个新成员，并且是首次在橡胶棒孢炭疽菌中发现。
