De Bortoli Caroline P, Santos Rafael F, Assirati Giordano J, Sun Xiaocun, Hietala Lucas, Jurat-Fuentes Juan Luis
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA.
Research Computing Support, Office of Information Technology, University of Tennessee, Knoxville, TN 37996, USA.
Insects. 2023 Dec 22;15(1):7. doi: 10.3390/insects15010007.
The fall armyworm (JE Smith) () is a polyphagous pest targeted by selected Cry and Vip3A insecticidal proteins from the bacterium (Bt) that are produced in transgenic Bt corn and cotton. Available evidence suggests that sublethal larval exposure to Cry1Ac increases flight activity in adult spp. However, it is not known whether this effect is also observed in survivors from generally lethal exposure to Cry1Ac. Moreover, while multiple cases of field-evolved resistance to Bt proteins have been described in the native range of , the effect of resistance on flight behavior has not been examined. Long-distance migratory flight capacity of is of concern given its ongoing global spread and the possibility that migrants may be carrying resistance alleles against pesticides and Bt crops. In this study, we used rotational flight mills to test the effects of generally lethal exposure to Cry1Ac in susceptible and sublethal exposure in Cry1F-resistant strains. The results detected altered pupal weight after larval feeding on diet containing Cry proteins, which only translated in significantly increased tendency for longer flights in female moths from the susceptible strain. This information has relevant implications when considering current models and assumptions for resistance management of Bt crops.
草地贪夜蛾（杰·史密斯）是一种多食性害虫，可被来自苏云金芽孢杆菌（Bt）的特定Cry和Vip3A杀虫蛋白靶向，这些蛋白在转基因Bt玉米和棉花中产生。现有证据表明，幼虫亚致死剂量接触Cry1Ac会增加成年夜蛾科物种的飞行活动。然而，尚不清楚在通常致死剂量接触Cry1Ac的幸存者中是否也观察到这种效应。此外，虽然在草地贪夜蛾的原生范围内已经描述了多起对Bt蛋白的田间进化抗性案例，但抗性对飞行行为的影响尚未得到研究。鉴于草地贪夜蛾在全球范围内的持续传播以及迁徙者可能携带抗农药和Bt作物抗性等位基因的可能性，其远距离迁飞能力备受关注。在本研究中，我们使用旋转飞行磨测试了通常致死剂量接触Cry1Ac对敏感品系和Cry1F抗性品系亚致死剂量接触的影响。结果发现，幼虫取食含Cry蛋白的饲料后，蛹重发生了变化，这仅转化为敏感品系雌蛾飞行时间显著增加的趋势。在考虑当前Bt作物抗性管理的模型和假设时，这些信息具有相关意义。
