Department of Epidemiology, University of Florida, Gainesville, Florida, USA.
Department of Pathology, University of Florida, Gainesville, Florida, USA.
Cannabis Cannabinoid Res. 2024 Aug;9(4):1028-1037. doi: 10.1089/can.2023.0139. Epub 2024 Jan 22.
HIV-related comorbidities appear to be related to chronic inflammation, a condition characterizing people living with HIV (PLWH). Prior work indicates that cannabidiol (CBD) might reduce inflammation; however, the genetics underpinning of this effect are not well investigated. Our main objective is to detect gene expression alterations in human peripheral blood mononuclear cells (PBMCs) from PLWH after at least 1 month of CBD treatment. We analyzed ∼41,000 PBMCs from three PLWH at baseline and after CBD treatment (27-60 days) through single-cell RNA sequencing. We obtained a coherent signature, characterized by an anti-inflammatory activity, of differentially expressed genes in myeloid cells. Our study shows how CBD is associated with alterations of gene expression in myeloid cells after CBD treatment. Clinical Trial Registration: NCT05209867.
HIV 相关合并症似乎与慢性炎症有关，这种情况发生在 HIV 感染者（PLWH）身上。先前的研究表明，大麻二酚（CBD）可能具有减轻炎症的作用；然而，其作用的遗传基础尚未得到充分研究。我们的主要目的是在至少接受 CBD 治疗 1 个月后，检测 PLWH 外周血单个核细胞（PBMC）中的基因表达变化。我们通过单细胞 RNA 测序分析了来自 3 名 PLWH 的约 41,000 个 PBMC，这些人在基线和 CBD 治疗后（27-60 天）。我们获得了一个一致的特征，表现为髓系细胞中差异表达基因的抗炎活性。我们的研究表明，在 CBD 治疗后，CBD 如何与髓系细胞中基因表达的改变相关。临床试验注册：NCT05209867。
