Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2024 Mar;395:130358. doi: 10.1016/j.biortech.2024.130358. Epub 2024 Jan 20.
This study investigated an innovative strategy of incorporating surfactants into alkaline-catalyzed glycerol pretreatment and enzymatic hydrolysis to improve lignocellulosic biomass (LCB) conversion efficiency. Results revealed that adding 40 mg/g PEG 4000 to the pretreatment at 195 °C obtained the highest glucose yield (84.6%). This yield was comparable to that achieved without surfactants at a higher temperature (240 °C), indicating a reduction of 18.8% in the required heat input. Subsequently, Triton X-100 addition during enzymatic hydrolysis of PEG 4000-assisted pretreated substrate increased glucose yields to 92.1% at 6 FPU/g enzyme loading. High-solid fed-batch semi-simultaneous saccharification and co-fermentation using this dual surfactant strategy gave 56.4 g/L ethanol and a positive net energy gain of 1.4 MJ/kg. Significantly, dual assistance with surfactants rendered 56.3% enzyme cost savings compared to controls without surfactants. Therefore, the proposed surfactant dual-assisted promising approach opens the gateway to economically viable enzyme-mediated LCB biorefinery.
本研究探讨了一种将表面活性剂纳入碱性甘油预处理和酶解中的创新策略，以提高木质纤维素生物质（LCB）的转化效率。结果表明，在 195°C 下添加 40mg/g 的聚乙二醇 4000 到预处理中可获得最高的葡萄糖产量（84.6%）。这一产量与在更高温度（240°C）下不使用表面活性剂时的产量相当，表明所需的热量输入减少了 18.8%。随后，在添加聚乙二醇 4000 辅助预处理底物的酶解过程中添加 Triton X-100，可将葡萄糖产量提高至 6 FPU/g 酶用量时的 92.1%。使用这种双重表面活性剂策略进行高固体分批补料半同步糖化和共发酵，可得到 56.4g/L 的乙醇和 1.4MJ/kg 的正净能量增益。值得注意的是，与不使用表面活性剂的对照相比，双重表面活性剂的辅助作用可节省 56.3%的酶成本。因此，所提出的表面活性剂双重辅助的有前途的方法为经济可行的酶介导的 LCB 生物精炼开辟了道路。
