Carneiro Deisy Guimarães, Pereira Aguilar Ananda, Mantovani Hilário Cuquetto, Mendes Tiago Antônio de Oliveira, Vanetti Maria Cristina Dantas
Department of Microbiology, Universidade Federal de Viçosa (UFV), Viçosa, Brazil.
Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa (UFV), Viçosa, Brazil.
Biofouling. 2024 Jan;40(1):14-25. doi: 10.1080/08927014.2024.2305385. Epub 2024 Jan 22.
Acyl-homoserine lactones (AHLs) are quorum-sensing signaling molecules in Gram-negative bacteria and positively regulate biofilm formation in under specific conditions. In this study, biofilm formation in was evaluated at 28 and 37 °C, under aerobic and anaerobic conditions. Additionally, the influence of the -dodecanoyl-DL-homoserine lactone (C12-HSL) on biofilm formation and the expression of genes related to the synthesis of structural components, regulation, and quorum sensing was assessed under anaerobiosis at 28 and 37 °C. Biofilm formation was found not to be influenced by the atmospheric conditions at 28 °C. However, it was reduced at 37 °C under anaerobiosis. C12-HSL enhanced biofilm formation at 37 °C under anaerobiosis and increased the expression of the and genes, suggesting an increase in c-di-GMP, a second messenger that controls essential physiological functions in bacteria. These results provide new insights into the regulation of biofilm formation in under anaerobic conditions.
酰基高丝氨酸内酯（AHLs）是革兰氏阴性菌中的群体感应信号分子，在特定条件下可正向调控生物膜形成。在本研究中，在28℃和37℃、需氧和厌氧条件下评估了[具体细菌名称未给出]中的生物膜形成情况。此外，在28℃和37℃厌氧条件下，评估了十二烷酰-DL-高丝氨酸内酯（C12-HSL）对生物膜形成以及与结构成分合成、调控和群体感应相关基因表达的影响。结果发现，28℃时生物膜形成不受大气条件影响。然而，在37℃厌氧条件下生物膜形成减少。C12-HSL在37℃厌氧条件下增强了生物膜形成，并增加了[具体基因名称未给出]和[具体基因名称未给出]基因的表达，这表明作为控制细菌基本生理功能的第二信使的环二鸟苷酸（c-di-GMP）增加。这些结果为厌氧条件下[具体细菌名称未给出]中生物膜形成的调控提供了新的见解。
