Panya Marutpong, Kaewraemruaen Chamraj, Saenwang Phairo, Pimboon Patcharin
Research Group for Biomedical Research and Innovative Development (RG-BRID), College of Medicine and Public Health, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, Thailand.
Department of Science and Bioinnovation, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Foods. 2024 Jan 16;13(2):287. doi: 10.3390/foods13020287.
Edible mushrooms, including wild mushrooms, are currently being investigated as natural sources to evaluate their prebiotic potential. This study aimed to evaluate the prebiotic potential of crude polysaccharides (CPSs) extracted from wild UBU_LS1 and UBU_LP2 and their application as cryoprotectants in the freeze-drying process to formulate a novel synbiotic product. Based on fruiting body morphology and molecular identification, two wild edible mushrooms named UBU_LS1 and UBU_LP2 were identified as and respectively. UBU_LS1 and UBU_LP2 contained high amounts of CPS after hot water extraction. Monosaccharide component analysis showed that CPS_UBU_LS1 and CPS_UBU_LP2 were typical heteropolysaccharides. CPS_UBU_LS1 and CPS_UBU_LP2 showed hydrolysis tolerance to the simulated human gastric acidic pH solution, indicating that these CPSs are capable of reaching the lower gastrointestinal tract. Antioxidant activity determined using the 1,1-diphenyl-2-picrylhydrazyl assay revealed that the CPS_UBU_LS1 and CPS_UBU_LP2 displayed greater antioxidant activity comparable with that of ascorbic acid. It was found that CPS_UBU_LS1 and CPS_UBU_LP2 have a high potential for stimulating growth in all probiotic strains. Moreover, both CPS compounds could possibly be used as cryoprotectants in freeze drying, since the viability of the selected probiotic 47-7 exhibited cell survival of greater than 70% after 90 days of storage at 4 °C. These results highlight that wild edible mushrooms UBU_LS1 and UBU_LP2 are potential natural sources of prebiotics and can be applied as cryoprotectants in the freeze-drying process. The crude polysaccharide derived from this study could also be considered as a potent antioxidative compound. Therefore, our study provides evidence to support the application of CPSs from wild edible mushrooms in synbiotic product development and in various functional foods. Finally, further evaluation of these prebiotics, including the determination of the potential rehabilitation of beneficial gut microbes in diseased individuals, is currently being conducted by our research group.
包括野生蘑菇在内的可食用蘑菇，目前正作为天然来源进行研究，以评估其益生元潜力。本研究旨在评估从野生UBU_LS1和UBU_LP2中提取的粗多糖（CPSs）的益生元潜力，以及它们在冷冻干燥过程中作为冷冻保护剂的应用，以制备一种新型合生元产品。基于子实体形态和分子鉴定，两种名为UBU_LS1和UBU_LP2的野生可食用蘑菇分别被鉴定为[此处原文缺失具体名称]和[此处原文缺失具体名称]。热水提取后，UBU_LS1和UBU_LP2含有大量的CPS。单糖成分分析表明，CPS_UBU_LS1和CPS_UBU_LP2是典型的杂多糖。CPS_UBU_LS1和CPS_UBU_LP2对模拟人体胃酸pH溶液具有水解耐受性，表明这些CPS能够到达下消化道。使用1,1-二苯基-2-苦基肼法测定的抗氧化活性表明，CPS_UBU_LS1和CPS_UBU_LP2表现出与抗坏血酸相当的更大抗氧化活性。发现CPS_UBU_LS1和CPS_UBU_LP2在刺激所有益生菌菌株生长方面具有很高的潜力。此外，两种CPS化合物都可能用作冷冻干燥中的冷冻保护剂，因为所选益生菌47-7在4℃储存90天后，细胞存活率大于70%。这些结果突出表明，野生可食用蘑菇UBU_LS1和UBU_LP2是益生元的潜在天然来源，可在冷冻干燥过程中用作冷冻保护剂。本研究中获得的粗多糖也可被视为一种有效的抗氧化化合物。因此，我们的研究为支持野生可食用蘑菇中的CPSs在合生元产品开发和各种功能性食品中的应用提供了证据。最后，我们的研究小组目前正在对这些益生元进行进一步评估，包括确定患病个体中有益肠道微生物的潜在恢复情况。
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