Abiodun Oyindamola Olajumoke, Alege Adenike Martha, Ezurike Precious Ulonnam, Nkumah Abraham, Adelowo Oluwatosin, Oke Tolulope Aderinola
University of Ibadan, Faculty of Basic Medical Sciences, Department of Pharmacology and Therapeutics, Ibadan, Nigeria.
University of Ibadan, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Ibadan, Nigeria.
Turk J Pharm Sci. 2022 Dec 21;19(6):642-648. doi: 10.4274/tjps.galenos.2021.72798.
Mushrooms are fungi with nutritional and health benefits. Mont., an edible fungus, has traditional usage and relevance in local therapy for managing metabolic diseases. In that view, this study aimed to evaluate the anti-obesity, anti-diabetic, and cytotoxic potential of the chloroform/methanol extract (CME) and aqueous extract (AE) of the mushroom.
was identified using molecular biology tools. The CME and AE were obtained sequentially and, then, subjected to α-amylase, α-glucosidase, and lipase inhibitory enzyme assays as well as total phenolic content (TPC) and flavonoid content (TFC) estimations. The cytotoxic potential of extract fractions of was assessed using the brine shrimp lethality assay.
The molecular identification of the mushroom displayed that the internal transcribed spacer sequence was an equivalent match to that of with a high percentage similarity, and thus assigned a unique accession number (KT120043.1). The CME of had higher TPC, TFC, and α-glucosidase inhibitory activity than AE. Furthermore, AE of the mushroom showed a higher lipase inhibitory potential with an IC value of 22.28 ± 0.65 μg/mL than the CME, while that of the reference, orlistat was 2.28 ± 0.34 μg/mL. However, these extracts exhibited very low or no α-amylase inhibitory and cytotoxic activity at the tested concentrations.
This study reveals that CME of , rich in polyphenols and flavonoids, possesses considerable α-glucosidase and lipase inhibitory activities.
蘑菇是具有营养和健康益处的真菌。蒙氏蘑菇是一种可食用真菌，在当地治疗代谢性疾病方面有传统用途和相关性。鉴于此，本研究旨在评估该蘑菇的氯仿/甲醇提取物（CME）和水提取物（AE）的抗肥胖、抗糖尿病和细胞毒性潜力。
使用分子生物学工具进行鉴定。依次获得CME和AE，然后进行α-淀粉酶、α-葡萄糖苷酶和脂肪酶抑制酶活性测定以及总酚含量（TPC）和黄酮含量（TFC）测定。使用卤虫致死试验评估该蘑菇提取物馏分的细胞毒性潜力。
该蘑菇的分子鉴定显示，其内部转录间隔区序列与[具体名称未给出]的序列高度相似，具有很高的百分比相似度，因此被赋予了一个独特的登录号（KT120043.1）。该蘑菇的CME比AE具有更高的TPC、TFC和α-葡萄糖苷酶抑制活性。此外，该蘑菇的AE显示出比CME更高的脂肪酶抑制潜力，IC50值为22.28±0.65μg/mL，而参考药物奥利司他的IC50值为2.28±0.34μg/mL。然而，在测试浓度下，这些提取物表现出极低的α-淀粉酶抑制活性或无α-淀粉酶抑制活性以及细胞毒性活性。
本研究表明，富含多酚和黄酮类化合物的该蘑菇的CME具有相当的α-葡萄糖苷酶和脂肪酶抑制活性。
