Yang Decao, Sun Panxi, Chen Yong, Jin Haojie, Xu Baohui, Ma Qingbian, Xue Lixiang, Wang Yan
Institute of Medical Innovation and Research, Peking University Third Hospital, Beijing 100191, China.
Medical Research Centre, Peking University Third Hospital, Beijing 100191, China.
Biomedicines. 2024 Jan 16;12(1):195. doi: 10.3390/biomedicines12010195.
It has been estimated that one in four stroke patients may have recurrent stroke within five years after they experienced the first stroke. Furthermore, clinical studies have shown that recurrent stroke negatively affects patient outcomes; the risk of disability and the death rate increase with each recurrent stroke. Therefore, it is urgent to find effective methods to prevent recurrent stroke. The gut microbiota has been proven to play an essential role after ischemic stroke, while sudden ischemia disrupts microbial dysbiosis, and the metabolites secreted by the microbiota also reshape the gut microenvironment. In the present study, we established a recurrent ischemic mouse model. Using this experimental model, we compared the survival rate and ischemic infarction between single MCAO and recurrent MCAO, showing that, when two surgeries were performed, the mouse survival rate dramatically decreased, while the infarction size increased. Fecal samples were collected on day 1, day 3 and day 7 after the first MCAO and day 9 (2 days after the second MCAO) for 16S sequencing, which provided a relatively comprehensive picture of the microbiota changes. By further analyzing the potential metabolic pathways, our data also highlighted several important pathways that were significantly altered after the first and recurrent stroke. In the present study, using an experimental mouse model, we showed that acute ischemic stroke, especially recurrent ischemia, significantly decreased the diversity of the gut microbiota.
据估计，四分之一的中风患者在首次中风后的五年内可能会复发中风。此外，临床研究表明，复发性中风会对患者的预后产生负面影响；每次复发性中风都会增加残疾风险和死亡率。因此，迫切需要找到有效的方法来预防复发性中风。肠道微生物群已被证明在缺血性中风后起着至关重要的作用，而突然的缺血会破坏微生物失调，微生物群分泌的代谢产物也会重塑肠道微环境。在本研究中，我们建立了复发性缺血小鼠模型。使用这个实验模型，我们比较了单次大脑中动脉闭塞（MCAO）和复发性MCAO之间的存活率和缺血性梗死情况，结果表明，进行两次手术时，小鼠存活率显著降低，而梗死面积增加。在首次MCAO后的第1天、第3天和第7天以及第9天（第二次MCAO后2天）收集粪便样本进行16S测序，这提供了微生物群变化的相对全面的情况。通过进一步分析潜在的代谢途径，我们的数据还突出了首次中风和复发性中风后显著改变的几个重要途径。在本研究中，我们使用实验小鼠模型表明，急性缺血性中风，尤其是复发性缺血，会显著降低肠道微生物群的多样性。
